Aberrant methylation of gene associated CpG sites occurs in borderline personality disorder
| Autor: | Reinhard Dammann, Stefanie Teschler, Christian Schmidt, Marek Bartkuhn, Gerhard Dammann, Natascha Künzel, Steffen Kiehl |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
medicine.medical_specialty lcsh:Medicine Biology Bioinformatics Pathogenesis Borderline Personality Disorder Internal medicine mental disorders medicine Humans Epigenetics lcsh:Science Borderline personality disorder Gene Oligonucleotide Array Sequence Analysis Multidisciplinary lcsh:R Case-control study Methylation Genomics DNA Methylation medicine.disease Endocrinology CpG site Case-Control Studies DNA methylation CpG Islands Female lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 12, p e84180 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Borderline personality disorder (BPD) is a complex psychiatric disease with an increased impact in the last years. While the diagnosis and therapy are well established, little is known on the pathogenesis of borderline personality disorder. Previously, a significant increase in DNA methylation of relevant neuropsychiatric genes in BPD patients has been reported. In our study we performed genome wide methylation analysis and revealed specific CpG sites that exhibited increased methylation in 24 female BPD patients compared to 11 female healthy controls. Bead chip technology and quantitative bisulfite pyrosequencing showed a significantly increased methylation at CpG sites of APBA2 (1.1 fold) and APBA3 (1.1 fold), KCNQ1 (1.5 fold), MCF2 (1.1 fold) and NINJ2 (1.2 fold) in BPD patients. For the CpG sites of GATA4 and HLCS an increase in DNA methylation was observed, but was only significant in the bead chip assay. Moreover genome wide methylation levels of blood samples of BPD patients and control samples are similar. In summary, our results show a significant 1.26 fold average increase in methylation at the analyzed gene associated CpG sites in the blood of BPD patients compared to controls samples (p |
| Databáze: | OpenAIRE |
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