Effects of β-d-xyloside on differentiation of the respiratory epithelium in the fetal mouse lung
| Autor: | Michael D. Allodoli, Robert L. Searls, S. Robert Hilfer, Candyce I. Smith, Mark A. Nathanson |
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| Rok vydání: | 1990 |
| Předmět: |
Cellular differentiation
Biology Extracellular matrix Mice Organ Culture Techniques medicine Animals Glycosides Respiratory system Lung Molecular Biology Dose-Response Relationship Drug Sulfates Cell Differentiation Epithelial Cells Cell Biology Epithelium Extracellular Matrix Cell biology Microscopy Electron medicine.anatomical_structure Proteoglycan Immunology Phosphatidylcholines biology.protein Respiratory epithelium Proteoglycans Basal lamina Developmental Biology |
| Zdroj: | Developmental Biology. 138:42-52 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(90)90175-i |
| Popis: | Differentiation of respiratory endings in the fetal lung appears to be controlled by its surrounding mesodermal capsule. The capsule may exert its influence by controlling the composition of the epithelial basal lamina or of the extended extracellular matrix that is deposited during the period when alveolar sacs are formed. As a first step in testing this hypothesis, the effects of the drug, rho-nitrophenyl-beta-D- xylopyranoside (beta-xyloside), an inhibitor of proteoglycan synthesis, and its inactive alpha anomer (alpha-xyloside) were examined. Lung primordia from mice at 16 days of gestation were tested for inhibition of morphological and functional differentiation as a result of drug treatment. Pseudoglandular lung epithelium did not form respiratory endings, contained fewer specialized cells, and accumulated little additional surfactant when treated with beta-xyloside but developed normally when treated with alpha-xyloside or grown in control medium. The results are interpreted to suggest that deposition of an extracellular matrix rich in proteoglycan is required to support maturation of the respiratory epithelium. |
| Databáze: | OpenAIRE |
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