Sedimentation analysis of von Willebrand and factor VIIIC protein using partition cells in the analytical ultracentrifuge
| Autor: | G H, Barlow, S E, Martin, V J, Marder |
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| Rok vydání: | 1984 |
| Předmět: | |
| Zdroj: | Blood. 63:940-943 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v63.4.940.bloodjournal634940 |
| Popis: | Sedimentation analysis of factor VIII complex was performed in the analytical ultracentrifuge using partition cells. This method allowed for the calculation of three different sedimentation coefficients from each run: one based on ristocetin agglutination activity for von Willebrand protein, SWF; one based on coagulant activity for factor VIIIC, SVIIIC; and one based on the schlieren or adsorption data for protein concentration, Sconc. In most cases, there was no agreement between the three values calculated from the same run, indicating a heterogeneous system. The calculated functional sedimentation coefficients give values that require the molecules to be highly asymmetric to be consistent with a glycoprotein of high molecular weight, which is in agreement with results observed in electron microscope studies. The dissociation of VIIIC into a smaller form can be demonstrated by this method. Determination of the three sedimentation coefficients in a series of fractions from gel filtration indicates a uniform size for the VIIIC activity but not for the WF activity. These observations are in agreement with the concept of a copolymer between WF and VIIIC and also with the concept of separate polymers for the two activities. |
| Databáze: | OpenAIRE |
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