Assessment of ischemic vascular damage
| Autor: | Ruzena Sotnikova, V. Nosalova |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ischemia
Drug Evaluation Preclinical Ileum Vascular permeability Toxicology Severity of Illness Index Mesenteric Artery Superior medicine.artery Occlusion Mesenteric Vascular Occlusion medicine Animals Superior mesenteric artery Vascular Diseases chemistry.chemical_classification Reactive oxygen species business.industry Ischemic injury medicine.disease Pathophysiology Rats Intestines Disease Models Animal medicine.anatomical_structure chemistry Anesthesia Reperfusion Injury business |
| Zdroj: | Current Protocols in Toxicology |
| ISSN: | 1934-9262 |
| Popis: | This protocol describes a model convenient for acute experiments in anesthetized rats, performed by selective occlusion of the superior mesenteric artery. Such a model provides a means for assessing the role played by various pathophysiological mechanisms in the development of intestinal ischemic injury. It is especially suitable for studying different treatments, mainly pharmacological ones, to help cope with this problem in clinical practice. In the form of support protocols, this unit includes functional vascular and chemiluminescence studies, determination of vascular permeability and myeloperoxidase activity, transit time, and mortality. Curr. Protoc. Toxicol. 44:21.4.1-21.4.21. © 2010 by John Wiley & Sons, Inc. Keywords: ischemia; reperfusion; reactive oxygen species; rat; superior mesenteric artery; ileum |
| Databáze: | OpenAIRE |
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