Physarum polycephalummutants in the photocontrol of sporulation display altered patterns in the correlated expression of developmentally regulated genes

Autor:	Viktoria Rätzel, Xenia-Katharina Hoffmann, Wolfgang Marwan, Britta Ebeling, Jens Tesmer
Rok vydání:	2013
Předmět:	Genetics Regulation of gene expression biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cellular differentiation Genes Protozoan Spores Protozoan Mutant Protozoan Proteins Gene Expression Regulation Developmental Physarum polycephalum Cell Biology biology.organism_classification Gene expression profiling Multinucleate Mutation Gene expression Gene Regulatory Networks Gene Developmental Biology
Zdroj:	Development, Growth & Differentiation. 55:247-259
ISSN:	0012-1592
DOI:	10.1111/dgd.12029
Popis:	Physarum polycephalum is a lower eukaryote belonging to the amoebozoa group of organisms that forms macroscopic, multinucleate plasmodial cells during its developmental cycle. Plasmodia can exit proliferative growth and differentiate by forming fruiting bodies containing mononucleate, haploid spores. This process, called sporulation, is controlled by starvation and visible light. To genetically dissect the regulatory control of the commitment to sporulation, we have isolated plasmodial mutants that are altered in the photocontrol of sporulation in a phenotypic screen of N-ethyl-N-nitrosourea (ENU) mutagenized cells. Several non-sporulating mutants were analyzed by measuring the light-induced change in the expression pattern of a set of 35 genes using GeXP multiplex reverse transcription-polymerase chain reaction with RNA isolated from individual plasmodial cells. Mutants showed altered patterns of differentially regulated genes in response to light stimulation. Some genes clearly displayed pairwise correlation in terms of their expression level as measured in individual plasmodial cells. The pattern of pairwise correlation differed in various mutants, suggesting that different upstream regulators were disabled in the different mutants. We propose that patterns of pairwise correlation in gene expression might be useful to infer the underlying gene regulatory network.
Databáze:	OpenAIRE
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