Isolation of Genomic Sequence Encoding a Biologically Active Bovine TGF- α Protein
| Autor: | Jacob S. Tou, M.F. McGrath, Zupec Me, Linda L. Zurfluh, Bolten Sl, Krivi Gg, John C. Byatt |
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| Rok vydání: | 1990 |
| Předmět: |
TGF alpha
Molecular Sequence Data Restriction Mapping Clinical Biochemistry Biology Kidney Polymerase Chain Reaction Salivary Glands Radioligand Assay Endocrinology Sequence Homology Nucleic Acid Animals Humans Amino Acid Sequence Northern blot chemistry.chemical_classification Genomic Library Base Sequence Cell growth Cell Membrane Nucleic acid sequence Nucleic Acid Hybridization RNA Biological activity DNA Exons Cell Biology Transforming Growth Factor alpha TGF beta receptor 2 Blotting Northern Molecular biology Rats Amino acid ErbB Receptors Liver chemistry Cattle Female Oligonucleotide Probes |
| Zdroj: | Growth Factors. 3:257-266 |
| ISSN: | 1029-2292 0897-7194 |
| Popis: | Genomic clones encoding bovine TGF-alpha were identified by hybridization with probes derived from human TGF-alpha sequence. Nucleotide sequence of the clones predicts that mature bovine TGF-alpha is a 50 amino acid polypeptide which shares 96% and 92% homology with human and rat TGF-alpha, respectively. Bovine TGF-alpha with the predicted sequence was chemically synthesized and tested for activity. Synthetic bovine TGF-alpha competes in a radioreceptor assay with labelled mouse EGF with activity parallel to that of human TGF-alpha and mouse EGF. The mitogenic activity of bovine TGF-alpha is comparable to that of human EGF in causing proliferation of bovine mammary epithelial cells. An approximately 5.0 kilobase RNA transcript is observed in polyadenylated RNA from MDBK cells by Northern blot analysis. The polymerase chain reaction detects the presence of a TGF-alpha transcript in many bovine tissues. These data indicate that bovine TGF-alpha may be a normal regulator of cell growth in the bovine animal. |
| Databáze: | OpenAIRE |
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