Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse

Autor: Marcos Silveira Buckeridge, Eleni Gomes, Maria de Lourdes Teixeira de Moraes Polizeli, Ana Claudia Vici, Ana Sílvia de Almeida Scarcella, Alexandre Favarin Somera, Christiane da Costa Carreira Nunes
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
Chemical Phenomena
Surface Properties
Inorganic chemistry
Pharmaceutical Science
SACARIFICAÇÃO
Microscopy
Atomic Force
01 natural sciences
Article
Analytical Chemistry
lcsh:QD241-441
Matrix (chemical analysis)
03 medical and health sciences
chemistry.chemical_compound
Hydrolysis
lcsh:Organic chemistry
010608 biotechnology
Drug Discovery
Physical and Theoretical Chemistry
Cellulose
glycosyl-hydrolase
030304 developmental biology
Steam explosion
Ions
0303 health sciences
Ethanol
Organic Chemistry
Substrate (chemistry)
Discriminant Analysis
food and beverages
Ammonium oxalate
pretreatment
sugarcane bagasse
Saccharum
saccharification
chemistry
Chemistry (miscellaneous)
lithium
second-generation ethanol
Yield (chemistry)
surface ion distribution
Molecular Medicine
Bagasse
ToF-SIMS
Zdroj: Molecules
Volume 24
Issue 19
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Molecules, Vol 24, Iss 19, p 3614 (2019)
ISSN: 1420-3049
DOI: 10.3390/molecules24193614
Popis: Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification. Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails. Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns. Both increased the saccharification yield 1.74-fold. NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control. In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O&minus
available for ionic sputtering. These changes correlated to 1.90-fold decrease in saccharification yields. Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification. Apparently, it acts as a phase-dependent modulator of enzyme activity. Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used. However, there were correlations between the Li+ distribution patterns and the enzymatic activities that should to be shown.
Databáze: OpenAIRE
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