The Utility of Real-Time Quantitative Polymerase Chain Reaction Genotype Detection in the Diagnosis of Urinary Tract Infections in Children
| Autor: | Katherine Hebert, Michael U. Callaghan, Deepak Kamat, Danielle M. Garshott, Andrew M. Fribley, Ahmad Farooqi, Chelsey Yurkovich, Jon Felt |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty Pathology Genotype Urinary system Urine Real-Time Polymerase Chain Reaction medicine.disease_cause Sensitivity and Specificity Gastroenterology law.invention Cohort Studies 03 medical and health sciences 0302 clinical medicine law 030225 pediatrics Internal medicine Escherichia coli medicine Humans Prospective Studies 030212 general & internal medicine Prospective cohort study Escherichia coli Infections Polymerase chain reaction Colony-forming unit business.industry Infant Reproducibility of Results bacterial infections and mycoses Real-time polymerase chain reaction Urinary Tract Infections Pediatrics Perinatology and Child Health Female business |
| Zdroj: | Clinical Pediatrics. 56:912-919 |
| ISSN: | 1938-2707 0009-9228 |
| DOI: | 10.1177/0009922817706144 |
| Popis: | Urinary tract infections (UTIs) are the most common serious bacterial infection in children with significant morbidity with delayed diagnosis. Polymerase chain reaction (PCR) is very accurate in detecting bacteria and widely available, but has never been evaluated to detect UTIs in children. To assess the utility of PCR as a rapid diagnostic tool, we conducted a prospective cohort study of 193 urine samples from children younger than 36 months undergoing evaluation for UTI in the emergency department over a 10-month period. A quantification cycle (Cq) threshold of 26.15 identified all Escherichia coli positive samples with sensitivity and specificity of 100% and 99.5%, respectively (95% CI = 71.5%-100% and 97.9%-99.5%, respectively). A Cq threshold of 19.03 identified E coli infections100 000 colony forming units/mL with sensitivity and specificity of 100% (95% CI = 72.2%-100% and 98.6%-100%, respectively). PCR is very accurate in diagnosing E coli UTIs in young children and could be useful as a rapid diagnostic tool. |
| Databáze: | OpenAIRE |
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