Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts
| Autor: | Tsen-Jung Lin, Hsin-Chun Chen, Hsiu-Mei Chiang, Kuo-Ching Wen, I-Chen Shih |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
MAPK/ERK pathway
Cell Survival MAP Kinase Signaling System Ultraviolet Rays Photoaging p38 mitogen-activated protein kinases Matrix metalloproteinase Toxicology Magnoliaceae p38 Mitogen-Activated Protein Kinases Antioxidants chemistry.chemical_compound Hyaluronic acid medicine Humans Fluorometry Zymography Hyaluronic Acid Cells Cultured Skin integumentary system biology Plant Extracts Chemistry Polyphenols General Medicine Fibroblasts medicine.disease Molecular biology Skin Aging Up-Regulation Matrix Metalloproteinase 9 Biochemistry Mitogen-activated protein kinase biology.protein Matrix Metalloproteinase 3 Matrix Metalloproteinase 1 Signal transduction Signal Transduction Food Science |
| Zdroj: | Food and Chemical Toxicology. 50:4260-4269 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2012.08.018 |
| Popis: | Solar ultraviolet (UV) radiation can cause skin photoaging by inducing secretion of matrix metalloproteinases (MMPs). It has been reported that MMPs, especially MMP-1, -3 and -9, reduce elasticity of the dermis by degrading collagen. Polyphenols are a group of compounds that exist mainly in glycosides in the plants and they may transform to aglycone after hydrolysis. Polyphenols can inhibit MMP expression and elastase activity. In this study, we investigated the effects of Michelia alba extract (MAE) on expression and activity of MMPs in human skin fibroblast cultures after UVB exposure. The results showed that MAE and its hydrolysates (MAH) inhibited collagenase and elastase activities. In addition, MAE exhibited antioxidant activity, elevated hyaluronic acid content and inhibited UVB-induced MMP-1, MMP-3 and MMP-9 expression. In addition, the zymography assay revealed that MAE also inhibited MMP-9 activity. We also found that MAE inhibited UVB-induced ERK and JNK kinase but not p38 kinase expression, suggesting that MAE may regulate the UVB-induced expression of MMP-1, MMP-3 and MMP-9 via the ERK and JNK kinase pathway. MAE could restore total collagen synthesis reduced by UVB. The results also suggest that MAE treatment may prevent UVB-induced extracellular matrix damage by inhibiting the expression of MMP-1, MMP-3 and MMP-9 through the MAP kinase pathway. Our findings imply that MAE is an effective agent against UVB-induced photodamage. |
| Databáze: | OpenAIRE |
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