Overexpression and extra-mitochondrial localization of the chaperonin Hsp60 in ameloblastoma
| Autor: | Mariana Rodríguez-Vázquez, Carlos Licéaga-Escalera, Mario A. Rodríguez, Josué Zuriel Ortiz-García, Ana Laura Martínez-Ricardez, Javier Portilla-Robertson, Marcos Agustín Muñiz-Lino, Mineko Shibayama, Roberto Onner Cruz-Tapia |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Chaperonins Dentigerous Cyst Medicine (miscellaneous) Odontogenic Tumors Biology General Biochemistry Genetics and Molecular Biology Chaperonin Ameloblastoma Mitochondrial Proteins 03 medical and health sciences 0302 clinical medicine medicine Humans Cyst General Dentistry 030206 dentistry Chaperonin 60 medicine.disease Immunohistochemistry Dentigerous cyst Blot 030104 developmental biology Membrane protein HSP60 |
| Zdroj: | Journal of oral biosciences. 63(3) |
| ISSN: | 1880-3865 |
| Popis: | Objectives Ameloblastoma is an odontogenic neoplasm of the mandible and maxilla with various histological types and subtypes. It has been reported that some ameloblastomas could arise from dentigerous cyst walls; thus, the development of ameloblastoma from dentigerous cysts may be due to differential protein expression. Our aim was to identify a membrane protein that is differentially expressed in ameloblastomas with respect to dentigerous cysts. Methods We analyzed the SDS-PAGE profiles of membrane proteins from ameloblastomas and dentigerous cysts. The protein in a band present in the ameloblastoma sample, but apparently absent in the dentigerous cyst sample was identified via mass spectrometry as the chaperonin Hsp60. We used western blotting and immunohistochemistry to analyze its overexpression and localization in ameloblastoma. Results We found a differential band of 95 kDa in the membrane proteins of ameloblastoma. In this band, the chaperonin Hsp60 was identified, and its overexpression was corroborated using western blotting and immunohistochemistry. Hsp60 was localized in the plasma membrane of all ameloblastoma samples studied; in addition, it was found in the cell nucleus of the plexiform subtype of conventional ameloblastoma. Conclusions Our results suggest that Hsp60 may be involved in ameloblastoma development, and could therefore be a potential therapeutic target for ameloblastoma treatment. |
| Databáze: | OpenAIRE |
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