Effects of human transforming growth factors on topoisomerases from normal fibroblasts
| Autor: | Martin D. Crespi, Alberto Baldi, F.H. Medina, Guillermo R. Castro, J.A. Genovese |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Cell
Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Fetus Epidermal growth factor medicine Animals Humans General Pharmacology Toxicology and Pharmaceutics Fibroblast Melanoma chemistry.chemical_classification Epidermal Growth Factor Topoisomerase General Medicine Fibroblasts Molecular biology Rats Kinetics Enzyme medicine.anatomical_structure DNA Topoisomerases Type II chemistry DNA Topoisomerases Type I Transforming Growth Factors biology.protein Rabbits Type II topoisomerase DNA Transforming growth factor |
| Zdroj: | Life sciences. 43(25) |
| ISSN: | 0024-3205 |
| Popis: | Normal rat kidney fibroblasts (NRK-B F49) treated at transforming doses with a gamma-like TGF, partially purified from human melanoma, showed a 3 to 5 fold increase in DNA type II topoisomerase activity. A similar effect was observed using EGF and a partially purified alfa TGF from rabbit fetuses. DNA type I topoisomerase activity, from the same cells, was not significantly modified by treatment with these growth factors. Topoisomerase II stimulation was dependent on mRNA synthesis. The possible role of topoisomerase II in phenotypical cell transformation induced by TGF is discussed. |
| Databáze: | OpenAIRE |
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