Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
| Autor: | Changyong Song, Kevin S. Raines, Zhifeng Huang, Anders Madsen, Lutz Wiegart, Alan Pryor, Allan L. Chen, Rui Xu, Chien-Chun Chen, Federico Zontone, Daewoong Nam, Jose A. Rodriguez, Peter J. Bradley, Jianwei Miao, Huaidong Jiang, Yuriy Chushkin |
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| Přispěvatelé: | University of California [Los Angeles] (UCLA), University of California, Natl Sun Yat Sen Univ, Dept Phys, Kaohsiung 80424, Taiwan, Carl ZEISS Xray Microscopy Inc, Pleasanton, CA 94588 USA, Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China, Stanford University, Pohang Univ Sci & Technol, Dept Phys, Pohang 790784, South Korea, Brookhaven National Laboratory [Upton, NY] (BNL), U.S. Department of Energy [Washington] (DOE)-UT-Battelle, LLC-Stony Brook University [SUNY] (SBU), State University of New York (SUNY)-State University of New York (SUNY), European Xray Free Electron Laser, D-22761 Hamburg, Germany, European Synchrotron Radiation Facility (ESRF) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
three-dimensional imaging
Materials science FOS: Physical sciences Flux Neospora caninum Biochemistry Atomic Physical Chemistry law.invention Optics Particle and Plasma Physics law Radiation damage [CHIM]Chemical Sciences ddc:530 General Materials Science Nuclear Physics - Biological Physics Crystallography business.industry Resolution (electron density) 3D reconstruction Detector X-ray imaging Molecular coherent diffraction General Chemistry Condensed Matter Physics Coherent diffraction imaging Research Papers Biological Physics (physics.bio-ph) QD901-999 coherent diffractive imaging X-ray crystallography physics.bio-ph physics.optics Electron microscope three-dimensional cellular structure business cryo-CDI Optics (physics.optics) Physics - Optics Physical Chemistry (incl. Structural) |
| Zdroj: | International Union of Crystallography journal International Union of Crystallography journal, International Union of Crystallography 2015, 2, pp.575-583. ⟨10.1107/S205225251501235X⟩ IUCrJ, vol 2, iss Pt 5 'IUCrJ ', vol: 2, pages: 575-583 (2015) IUCrJ IUCrJ, Vol 2, Iss 5, Pp 575-583 (2015) IUCrJ 2(5), 575-583 (2015). doi:10.1107/S205225251501235X |
| ISSN: | 2052-2525 |
| DOI: | 10.1107/S205225251501235X⟩ |
| Popis: | Since its first experimental demonstration in 1999, coherent diffractive imaging (CDI) has been applied to image a broad range of samples using advanced synchrotron radiation, X-ray free-electron lasers, high harmonic generation and electrons. Here, the first experimental demonstration of cryogenic CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells is reported. As a proof of principle, the three-dimensional mass density of the sub-cellular organization of a Neospora caninum cell is determined based on its natural contrast. A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres. |
| Databáze: | OpenAIRE |
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