Nano-sized biopolymer levan: Its antimicrobial, anti-biofilm and anti-cancer effects
| Autor: | Nilüfer Cihangir, Gözde Koşarsoy Ağçeli |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pseudomonas mandelii
Antineoplastic Agents engineering.material 010402 general chemistry Polysaccharide 01 natural sciences Biochemistry Analytical Chemistry Biopolymers Fructan Tumor Cells Cultured Humans Yeast extract Food science Cell Proliferation chemistry.chemical_classification Bacteria biology 010405 organic chemistry Organic Chemistry Biofilm General Medicine biology.organism_classification Anti-Bacterial Agents Fructans 0104 chemical sciences chemistry Biofilms MCF-7 Cells engineering Nanoparticles Biopolymer Drug Screening Assays Antitumor Antibacterial activity |
| Zdroj: | Carbohydrate Research. 494:108068 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/j.carres.2020.108068 |
| Popis: | Among other polysaccharides, levan is a fructan with great potential in biotechnological applications due to its functional properties. The levan has only been available in small quantities because of its high cost. Here, a levan-producing microorganism was isolated from soil and identified as Pseudomonas mandelii. TGA, SEM, FTIR, 1H NMR, 13C NMR and Zetasizer analyses were used to characterize thermal properties and the morphology of the levan. It is the first time, the levan synthesized from P. mandelii is reported as nano sized. The culture conditions were optimized. It is the most comprehensive optimization study regarding levan production of P. mandelii up to the present. The optimum conditions found for levan production were 37 °C, at pH 8, under static conditions, with 15% sucrose and 1.5% mannitol as the C sources and yeast extract as the N source. In addition, the levan production yield was calculated at optimum conditions. Antibacterial activity of levan was evaluated against bacteria and the largest zone of inhibition was observed against E. coli at a concentration of 1000 μg/mL. Antibiofilm activity of levan was evaluated, and we found that all levan concentrations inhibited biofilm formation of all microorganisms in the study. We have shown that the levan from Pseudomonas mandelii induce cytotoxicity breast (MCF-7) cells in a dose-dependent manner. |
| Databáze: | OpenAIRE |
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