Enzyme sensor with polydimethylsiloxane membrane and CMOS potentiostat for wide-range glucose measurements
| Autor: | Holger Vogt, Stefan Mross, Michael Kraft, Peter Furst, Tom Zimmermann, Sebastien Pierrat |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Analytical chemistry
Materialtechnik bioreactor monitoring chemistry.chemical_compound PDMS Glucose oxidase Electrical and Electronic Engineering Instrumentation membrane Curing (chemistry) Elektrotechnik glucose sensor cellulose acetate Chromatography Polydimethylsiloxane biology Glucose Measurement CMOS potentiostat Cellulose acetate Potentiostat Membrane chemistry Electrode biology.protein high glucose concentrations wide range measurement |
| Popis: | We report on an electrochemical measurement setup comprising a glucose sensor and a CMOS potentiostat with a two-layer membrane as the first steps toward the development of an integrated in-situ sensor system for bioreactors. The potentiostat has a chip size of 2.1 mm × 2.5 mm and a linear current range from -220 nA to 240 nA with a linearity of R 2 = 0.9995. For wide range measurements of glucose concentrations in cell culture media, electrodes functionalized with the enzyme glucose oxidase were spin-coated with membranes made from polydimethylsiloxane (PDMS). A two-stage curing scheme of the PDMS was applied, and different membrane thicknesses and curing times were evaluated. With these membranes, glucose concentrations up to 500 mM were measured with a linear measurement range up to 200 mM. The sensors were successfully employed in the glucose monitoring of a culture of Saccharomyces cerevisiae to monitor the glucose consumption of the cells. For interference elimination, the cellulose acetate membranes were employed. |
| Databáze: | OpenAIRE |
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