Human elongation factor 1β: cDNA and derived amino acid sequence
| Autor: | Karl Heinz Scheit, M. Zimmer, H. von der Kammer, J. Klaudiny |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Biophysics Sequence alignment Biochemistry Peptide Elongation Factor 1 Sequence Homology Nucleic Acid Complementary DNA Animals Humans Genomic library Amino Acid Sequence Molecular Biology Peptide sequence Gene Library chemistry.chemical_classification Granulosa Cells Base Sequence Chemistry cDNA library Elongation Factor 1-Beta Nucleic acid sequence DNA Cell Biology Blotting Northern Peptide Elongation Factors Molecular biology Amino acid Ribonucleoproteins RNA Female Artemia |
| Zdroj: | Biochemical and Biophysical Research Communications. 177:312-317 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(91)91984-k |
| Popis: | From a cDNA library in lambda gt11 derived from poly (A+)RNA of human ovarian granulosa cells a cDNA clone lambda HGP34, containing an EcoRI insert of 829 bp, was identified. After subcloning of the insert into pUC18, the clone pHGP34 was obtained and sequenced. The derived amino acid sequence, corresponding to a protein of 225 amino acids, shows a high degree of homology to elongation factor 1 beta (EF-1 beta) of Artemia salina (57%) and known peptide sequences of Xenopus laevis EF-1 beta (86%). We therefore assume that the protein coded for by pHGP34 represents human EF-1 beta. Northern analysis reveals an EF-1 beta specific mRNA of 900 bp. Southern analysis indicates that EF-1 beta in the human genome, like EF-1 alpha, appears to be specified by more than one gene. A high degree of sequence homology for EF-1 beta specific sequences is observed for bovine, rat and mouse species. |
| Databáze: | OpenAIRE |
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