Bulked Segregant RNA-Seq Reveals Distinct Expression Profiling in Chinese Wheat Cultivar Jimai 23 Responding to Powdery Mildew
| Autor: | Ma Pengtao, Du Wenxiao, Wu Liru, Liu Jianjun, Han Ran, He Huagang, Jia Mengshu, Cheng Liu, Wang Wenrui, Jiancheng Song, Liancheng Liu, Li Haosheng, Zhang Xu, Liping Niu, Wang Xiaolu, Liang Xiao, Hongxing Xu, Tong Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
BSR-Seq lcsh:QH426-470 Blumeria graminis RNA-Seq Single-nucleotide polymorphism Biology 03 medical and health sciences 0302 clinical medicine wheat Genetics DEG Cultivar Gene Genetics (clinical) Original Research Chromosome food and beverages biology.organism_classification Gene expression profiling lcsh:Genetics 030104 developmental biology expression profiling 030220 oncology & carcinogenesis Molecular Medicine powdery mildew Powdery mildew |
| Zdroj: | Frontiers in Genetics Frontiers in Genetics, Vol 11 (2020) |
| ISSN: | 1664-8021 |
| Popis: | Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive fungal diseases threatening global wheat production. Host resistance is well known to be the most efficient method to control this disease. However, the molecular mechanism of wheat powdery mildew resistance (Pm) is still unclear. To analyze the molecular mechanism of Pm, we used the resistant wheat cultivar Jimai 23 to investigate its potential resistance components and profiled its expression in response to powdery mildew infection using bulked segregant RNA-Seq (BSR-Seq). We showed that the Pm of Jimai 23 was provided by a single dominant gene, tentatively designated PmJM23, and assigned it to the documented Pm2 region of chromosome 5DS. 3,816 consistently different SNPs were called between resistant and susceptible parents and the bulked pools derived from the combinations between the resistant parent Jimai23 and the susceptible parent Tainong18. 58 of the SNPs were assigned to the candidate region of PmJM23. Subsequently, 3,803 differentially expressed genes (DEGs) between parents and bulks were analyzed by GO, COG and KEGG pathway enrichment. The temporal expression patterns of associated genes following Bgt inoculation were further determined by RT-qPCR. Expression of six disease-related genes was investigated during Bgt infection and might serve as valuable genetic resources for the improvement of durable resistance to Bgt. |
| Databáze: | OpenAIRE |
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