Quantitative photoelectrochemical detection of biological affinity reaction: biotin-avidin interaction
| Autor: | Dong Zheng, Fuquan Wang, Liang-Hong Guo, Xiqiang Yang, Na Wang, Yuan-Guang Li, Jing Cheng, Dong Dong |
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| Rok vydání: | 2004 |
| Předmět: |
Light
Photochemistry Inorganic chemistry chemistry.chemical_element Biotin Electron donor Biosensing Techniques Binding Competitive Oxalate Ruthenium Analytical Chemistry chemistry.chemical_compound Bipyridine 2 2'-Dipyridyl Electrochemistry Colloids Bovine serum albumin Electrodes Photocurrent Oxalates biology Tin Compounds Serum Albumin Bovine Avidin chemistry biology.protein Protein Binding |
| Zdroj: | Analytical chemistry. 76(2) |
| ISSN: | 0003-2700 |
| Popis: | Quantitative detection of a biological affinity reaction, the biotin/avidin recognition, was achieved using our newly developed photoelectrochemical analytical system. The system is based on the operation mechanism of the well-developed dye-sensitized photoelectrochemical solar cells and comprises a ruthenium tris(2,2'-bipyridine) (Ru-bipy) derivative as the photoelectrochemical signal-generating molecule, oxalate as the sacrificial electron donor, and tin oxide nanoparticle as the semiconductor electrode material. To perform the affinity reaction, avidin was immobilized on SnO(2) electrode by passive adsorption. Biotin-linked bovine serum albumin (BSA) was labeled with an NHS-ester derivative of Ru-bipy. After binding of BSA to the surface-immobilized avidin through biotin, photoelectrochemical measurement was carried out in the presence of oxalate. Anodic photocurrent was turned on and off repeatedly by control of incidental light. The action spectrum of the photocurrent resembled the absorption spectrum of Ru-bipy, proving the photocurrent was generated from the metal complex. A linear relationship between photocurrent and BSA concentration was obtained in the range of 1-100 microg/mL. This is the first case of quantitative photoelectrochemical detection of a biological affinity interaction. |
| Databáze: | OpenAIRE |
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