The protein kinase TOUSLED facilitates RNAi inArabidopsis
| Autor: | William J. Lucas, Gregory Schott, Salina Akhter, Patrice Dunoyer, Chun-Lin Shi, Jae-Yean Kim, Olivier Voinnet, Mohammad Nazim Uddin |
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| Přispěvatelé: | Institute of Public Health, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Rok vydání: | 2014 |
| Předmět: |
Small RNA
Small interfering RNA RNA-induced transcriptional silencing RNA-induced silencing complex 1.1 Normal biological development and functioning Trans-acting siRNA Arabidopsis Genetically Modified Protein Serine-Threonine Kinases Biology Underpinning research Gene Expression Regulation Plant RNA interference Information and Computing Sciences Genetics RNA-Induced Silencing Complex ComputingMilieux_MISCELLANEOUS Arabidopsis Proteins RNA [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Plant Plants Biological Sciences Protein-Serine-Threonine Kinases Plants Genetically Modified Small Untranslated RNA silencing Gene Expression Regulation RNA Small Untranslated RNA Interference Environmental Sciences Biotechnology Developmental Biology |
| Zdroj: | Nucleic Acids Research, 42 (12) Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2014, 42 (12), pp.7971-7980 NUCLEIC ACIDS RESEARCH(42): 12 Nucleic acids research, vol 42, iss 12 |
| ISSN: | 1362-4962 0305-1048 0301-5610 |
| Popis: | RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that include antiviral defense, maintenance of genome integrity and the orchestration of cell fates. Although most generic or core components of the various plant small RNA pathways have been likely identified over the past 15 years, factors involved in RNAi regulation through post-translational modifications are just starting to emerge, mostly through forward genetic studies. A genetic screen designed to identify factors required for RNAi in Arabidopsis identified the serine/threonine protein kinase, TOUSLED (TSL). Mutations in TSL affect exogenous and virus-derived siRNA activity in a manner dependent upon its kinase activity. By contrast, despite their pleiotropic developmental phenotype, tsl mutants show no defect in biogenesis or activity of miRNA or endogenous trans-acting siRNA. These data suggest a possible role for TSL phosphorylation in the specific regulation of exogenous and antiviral RNA silencing in Arabidopsis and identify TSL as an intrinsic regulator of RNA interference. Nucleic Acids Research, 42 (12) ISSN:1362-4962 ISSN:0301-5610 |
| Databáze: | OpenAIRE |
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