Strong correspondence in bacterial loads between the vagina and rectum of pregnant women

Autor: Rita Verhelst, Nabil Abdullah El Aila, Inge Tency, Mario Vaneechoutte, Guido Lopes dos Santos Santiago, Marleen Temmerman, Ellen De Backer, Bart Saerens, Piet Cools, Hans Verstraelen
Rok vydání: 2011
Předmět:
Zdroj: Research in Microbiology. 162:506-513
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2011.04.004
Popis: We sampled the vagina and rectum in 71 pregnant women and bacterial loads of Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, Gardnerella vaginalis and Atopobium vaginae were determined by culture and quantitative PCR (qPCR). Culture and qPCR results differed substantially with regard to the evaluation of vaginal and rectal occurrence of the six species tested. The vaginal-rectal prevalence of L. crispatus, L. jensenii, L. gasseri, L. iners, G. vaginalis and A. vaginae as established by culture vs. PCR was 32.3 vs. 91.5%, 32.3 vs. 77.4%, 28.1 vs. 91.5%, 12.6 vs. 68.5%, 12.6 vs. 74.6% and 5.6 vs. 69.0%, respectively. Using qPCR, a significant positive correlation was found between vaginal and rectal loads of L. crispatus (p
Databáze: OpenAIRE