Interaction between paramagnetic metal complexes and intracellular water of single cells
| Autor: | Konrad Keller, Adolf Zschunke, Sabine Päuser |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Magnetic Resonance Spectroscopy
Cell Membrane Inulin Spin–lattice relaxation Water chemistry.chemical_element Anatomy Copper Metal Magnetics Xenopus laevis Membrane chemistry Cytoplasm visual_art Oocytes Biophysics visual_art.visual_art_medium Animals Molecule Radiology Nuclear Medicine and imaging Chelation Intracellular |
| Zdroj: | Journal of Magnetic Resonance Imaging. 6:250-254 |
| ISSN: | 1522-2586 1053-1807 |
| DOI: | 10.1002/jmri.1880060144 |
| Popis: | Oocytes from Xenopus laevis were used as a well-established model to investigate spatially resolved changes in relaxation time (T1) within a cell during exposure to copper complexes by means of 'H-NMR-microscopy. T1 relaxation of intracellular water was shortened in dependence on the complex concentration, the water content, and the water mobility in various cell compartments. A relatively constant T1 decrease was observed in the cytoplasm of the vegetal pole, irrespective of the type and concentration of the permeable complexes used. Since the lowest content of free mobile water molecules was detected at the vegetal pole, we concluded that this quantity was as important for the relaxation time decrease as that of the chelated paramagnetic ions. Experiments using 14C-labeled inulin demonstrated that the paramagnetic metal complexes entered the oocytes without gross injury to their plasma membranes. |
| Databáze: | OpenAIRE |
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