Prevalence and polymorphism of a mussel transmissible cancer in Europe

Autor: Guillaume M. Charrière, Maryline Houssin, Maurine Hammel, John J. Welch, Christine Arbiol, Michael J. Metzger, Ismaël Bernard, Nicolas Bierne, Delphine Destoumieux-Garzón, E. A. V. Burioli, Antonio Villalba, Jean-Francois Pepin, Abdellah Benabdelmouna, Alexis Simon, Jean-Baptiste Lamy
Přispěvatelé: Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Center for Applied Medical Research [Plamplona] (CIMA), Universidad de Navarra [Pamplona] (UNAV), Universidad de Alcala, Spain, University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Laboratoire Environnement Ressources des Pertuis Charentais (LERPC), LITTORAL (LITTORAL), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Santé, Génétique et Microbiologie des Mollusques (SGMM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), SAS Eurêka Mer, University of Cambridge [UK] (CAM), Pacific Northwest Research Institute (PNRI), ANR-18-CE35-0009,TRANSCAN,ECOLOGIE ET EVOLUTION DES CANCERS TRANSMISSIBLES(2018), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), CBET research group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PiE and Science and Technology Faculty, University of the Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain, Laboratoire Environnement Ressources des Pertuis Charentais (LER/PC), Santé, Génétique et Microbiologie des Mollusques (IFREMER SG2M), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), Hammel, Maurine [0000-0001-9937-5678], Simon, Alexis [0000-0002-6176-5045], Burioli, Erika AV [0000-0003-2666-9258], Pépin, Jean-François [0000-0001-8548-8938], Lamy, Jean-Baptiste [0000-0002-6078-0905], Bernard, Ismael [0000-0002-3666-7419], Charrière, Guillaume M [0000-0002-4796-1488], Destoumieux-Garzon, Delphine [0000-0002-8793-9138], Bierne, Nicolas [0000-0003-1856-3197], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Mol Ecol
Molecular Ecology
Molecular Ecology, 2022, 31 (3), pp.736-751. ⟨10.1111/mec.16052⟩
Molecular ecology, vol 31, iss 3
Molecular Ecology, Wiley, 2021, ⟨10.1111/mec.16052⟩
Molecular Ecology (0962-1083) (Wiley), 2022-02, Vol. 31, N. 3, P. 736-751
ISSN: 0962-1083
1365-294X
DOI: 10.1111/mec.16052⟩
Popis: Transmissible cancers are parasitic malignant cell lineages that have acquired the ability to infect new hosts from the same species, or sometimes related species. First described in dogs and Tasmanian devils, transmissible cancers were later discovered in some marine bivalves affected by a leukaemia-like disease. In Mytilus mussels, two lineages of bivalve transmissible neoplasia (BTN) have been described to date (MtrBTN1 and MtrBTN2), both of which emerged in a Mytilus trossulus founder individual. Here, we performed extensive screening of genetic chimerism, a hallmark of transmissible cancer, by genotyping 106single nucleotide polymorphisms of 5,907 European Mytilus mussels. Genetic analysis allowed us to simultaneously obtain the genotype of hosts - Mytilus edulis, M.galloprovincialis or hybrids - and the genotype of tumours of heavily infected individuals. In addition, a subset of 222 individuals were systematically genotyped and analysed by histology to screen for possible nontransmissible cancers. We detected MtrBTN2 at low prevalence in M. edulis, and also in M.galloprovincialis and hybrids although at a much lower prevalence. No MtrBTN1 or new BTN were found, but eight individuals with nontransmissible neoplasia were observed at a single polluted site on the same sampling date. We observed a diversity of MtrBTN2genotypes that appeared more introgressed or more ancestral than MtrBTN1 and reference healthy M.trossulus individuals. The observed polymorphism is probably due to somatic null alleles caused by structural variations or point mutations in primer-binding sites leading to enhanced detection of the host alleles. Despite low prevalence, two sublineages divergent by 10% fixed somatic null alleles and one nonsynonymous mtCOI (mitochondrial cytochrome oxidase I) substitution are cospreading in the same geographical area, suggesting a complex diversification of MtrBTN2since its emergence and host species shift.
Databáze: OpenAIRE
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