Body fat free mass is associated with the serum metabolite profile in a population-based study
Autor: | Thomas Illig, Jerzy Adamski, Fabian J. Theis, Karsten Suhre, Annette Peters, Jan Krumsiek, Cornelia Prehn, Elisabeth Altmaier, H.-Erich Wichmann, Angela Döring, Christa Meisinger, Gabi Kastenmüller, Christian Gieger, Werner Römisch-Margl, Jakob Linseisen, Carolin Jourdan, Ann-Kristin Petersen, Rui Wang-Sattler |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
medicine.medical_specialty
Anatomy and Physiology Epidemiology Metabolite Science Population Biochemistry chemistry.chemical_compound Metabolomics Internal medicine medicine Humans ddc:610 Obesity Carnitine education Biology Aged Nutrition chemistry.chemical_classification Molecular Epidemiology education.field_of_study Multidisciplinary Population Biology Fatty acid Metabolism Middle Aged Amino acid Biomarker Epidemiology Endocrinology Adipose Tissue chemistry Body Composition Medicine Physiological Processes Energy Metabolism Blood Chemical Analysis Drug metabolism Research Article medicine.drug |
Zdroj: | PLoS ONE 7:e40009 (2012) PLoS ONE, Vol 7, Iss 6, p e40009 (2012) PLoS ONE |
Popis: | ObjectiveTo characterise the influence of the fat free mass on the metabolite profile in serum samples from participants of the population-based KORA (Cooperative Health Research in the Region of Augsburg) S4 study.Subjects and methodsAnalyses were based on metabolite profile from 965 participants of the S4 and 890 weight-stable subjects of its seven-year follow-up study (KORA F4). 190 different serum metabolites were quantified in a targeted approach including amino acids, acylcarnitines, phosphatidylcholines (PCs), sphingomyelins and hexose. Associations between metabolite concentrations and the fat free mass index (FFMI) were analysed using adjusted linear regression models. To draw conclusions on enzymatic reactions, intra-metabolite class ratios were explored. Pairwise relationships among metabolites were investigated and illustrated by means of Gaussian graphical models (GGMs).ResultsWe found 339 significant associations between FFMI and various metabolites in KORA S4. Among the most prominent associations (p-values 4.75 × 10(-16)-8.95 × 10(-06)) with higher FFMI were increasing concentrations of the branched chained amino acids (BCAAs), ratios of BCAAs to glucogenic amino acids, and carnitine concentrations. For various PCs, a decrease in chain length or in saturation of the fatty acid moieties could be observed with increasing FFMI, as well as an overall shift from acyl-alkyl PCs to diacyl PCs. These findings were reproduced in KORA F4. The established GGMs supported the regression results and provided a comprehensive picture of the relationships between metabolites. In a sub-analysis, most of the discovered associations did not exist in obese subjects in contrast to non-obese subjects, possibly indicating derangements in skeletal muscle metabolism.ConclusionA set of serum metabolites strongly associated with FFMI was identified and a network explaining the relationships among metabolites was established. These results offer a novel and more complete picture of the FFMI effects on serum metabolites in a data-driven network. |
Databáze: | OpenAIRE |
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