A standardized extract of the fruit of Ziziphus jujuba (Jujube) induces neuronal differentiation of cultured PC12 cells: a signaling mediated by protein kinase A
| Autor: | Janis Y. X. Zhan, Jianping Chen, Kelly Y.C. Lam, Karl Wah Keung Tsim, Tina T. X. Dong, Maitinuer Maiwulanjiang, Ping Yao, Wendy L. Zhang, David T W Lau, Candy T.W. Lam, Sherry L. Xu, Kevin Y. Zhu |
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| Rok vydání: | 2014 |
| Předmět: |
CAMP Responsive Element Binding Protein
Neurite PC12 Cells food Animals Phosphorylation Protein kinase A Cyclic AMP Response Element-Binding Protein Neurons biology Kinase Cell Differentiation Ziziphus General Chemistry Cyclic AMP-Dependent Protein Kinases food.food Cell biology Rats Nerve growth factor Ziziphus jujuba Biochemistry Fruit biology.protein General Agricultural and Biological Sciences Neurotrophin Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Journal of agricultural and food chemistry. 62(8) |
| ISSN: | 1520-5118 |
| Popis: | The fruit of Ziziphus jujuba Mill., known as Chinese date or jujube, is consumed as a health supplement worldwide. To study the role of jujube in brain benefits, its effects on neuronal differentiation of PC12 cells were studied. Application of jujube water extract induced neurite outgrowth of PC12 cells, >25% of which were differentiated; this effect was similar to that of nerve growth factor. In parallel, the expressions of neurofilaments (NFs) in jujube-treated cultures showed a dose-dependent increase, with the highest inductions by ∼150% for NF68 and NF160 and by ∼100% for NF200. Application of H89, a protein kinase A inhibitor, attenuated jujube-induced neurite outgrowth of the cultures. Besides, using jujube extract induced the phosphorylation of cAMP responsive element binding protein on PC12 cells, which was blocked by H89. These results support the use of jujube as a food supplement for the prevention of neurodegenerative diseases in which neurotrophin deficiency is involved. |
| Databáze: | OpenAIRE |
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