Differential expression of neuron-specific enolase mRNA in mouse neuroblastoma cells in response to differentiation inducing agents
| Autor: | Daniele Oliva, Serafina Sciarrino, Agata Giallongo, Valeria Matranga, L. D’Amelio |
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| Rok vydání: | 1993 |
| Předmět: |
Neurite
Cellular differentiation Enolase Retinoic acid Tretinoin Biology Culture Media Serum-Free Gene Expression Regulation Enzymologic Mice Neuroblastoma Cellular and Molecular Neuroscience chemistry.chemical_compound Gene expression Tumor Cells Cultured medicine Animals Dimethyl Sulfoxide RNA Messenger RNA Neoplasm Northern blot Neurons Contact Inhibition Cell Differentiation Cell Biology General Medicine Blotting Northern medicine.disease Neoplasm Proteins Cell biology Gene Expression Regulation Neoplastic Isoenzymes Bucladesine chemistry Cell culture Phosphopyruvate Hydratase Immunology |
| Zdroj: | Cellular and Molecular Neurobiology. 13:137-145 |
| ISSN: | 1573-6830 0272-4340 |
| DOI: | 10.1007/bf00735370 |
| Popis: | SUMMARY 1. The mouse neuroblasma cell line N-115 was used as a model system to study neuronal differentiation induced by treatment of cells with different agents. 2. The extent of morphological differentiation obtained with dibutyryl cyclic AMP (dbc-AMP), dimethyl sulfoxide (DMSO), retinoic acid (RA), and serumfree medium was correlated to the expression of the mRNA for the gamma isoform of the glycolytic enzyme enolase, a recognized neuron-specific marker. 3. A 4-day treatment of the cells with any of the differentiation inducing agents used in this study resulted in the extension of long neurites, though differences in cell body shape were observed depending on the agent used. 4. Northern blot analysis revealed that changes in the level of gamma enolase-specific mRNA correlate with the extent of morphological differentiation, with a 5- to 20-fold increase depending on the differentiation inducing agent used. 5. Finally, we found that a high cell density causes a significative increase in the level of the gamma enolase-specific message in cells maintained in growing conditions. |
| Databáze: | OpenAIRE |
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