In vitro efficacy of a buffered chelating solution as an antimicrobial potentiator for antifungal drugs against fungal pathogens obtained from horses with mycotic keratitis
| Autor: | William L. Weinstein, Richard E. Wooley, Phillip A. Moore, Branson W. Ritchie, Ursula M. Dietrich, Susan Sanchez |
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| Rok vydání: | 2006 |
| Předmět: |
Antifungal Agents
Miconazole Natamycin Itraconazole Microbiology medicine Animals Horses Candida albicans Chelating Agents Keratitis General Veterinary biology Drug Synergism General Medicine biology.organism_classification Antimicrobial Paecilomyces variotii Solutions Ketoconazole Mycoses Penicillium Horse Diseases medicine.drug |
| Zdroj: | American Journal of Veterinary Research. 67:562-568 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.67.4.562 |
| Popis: | Objective—To determine whether a novel third-generation chelating agent (8mM disodium EDTA dehydrate and 20mM 2-amino-2-hydroxymethyl-1, 3-propanediol) would act as an antimicrobial potentiator to enhance in vitro activity of antifungal medications against fungal isolates obtained from horses with mycotic keratitis. Sample Population—Fungal isolates (3 Aspergillus isolates, 5 Fusarium isolates, 1 Penicillium isolate, 1 Cladosporium isolate, and 1 Curvularia isolate) obtained from horses with mycotic keratitis and 2 quality-control strains obtained from the American Type Culture Collection (ATCC; Candida albicans ATCC 90028 and Paecilomyces variotii ATCC 36257). Procedure—Minimum inhibitory concentrations (MICs) against fungal isolates for 4 antifungal drugs (miconazole, ketoconazole, itraconazole, and natamycin) were compared with MICs against fungal isolates for the combinations of each of the 4 antifungal drugs and the chelating agent. The Clinical and Laboratory Standards Institute microdilution assay method was performed by use of reference-grade antifungal powders against the fungal isolates and quality-control strains of fungi. Results—Values for the MIC at which the antifungal drugs decreased the growth of an organism by 50% (MIC50) and 90% (MIC90) were decreased for the control strains and ophthalmic fungal isolates by 50% to 100% when the drugs were used in combination with the chelating agent at a concentration of up to 540 μg/mL. Conclusions and Clinical Relevance—The chelating agent increased in vitro activity of antifungal drugs against common fungal pathogens isolated from eyes of horses with mycotic keratitis. |
| Databáze: | OpenAIRE |
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