Comparative and molecular analysis of MRSA isolates from infection sites and carrier colonization sites

Autor: Asim Diab, Fatimah I Alkhamis, Khaled R. Alkharsah, Amani Alnimr, Suriya Rehman, Amein K. Al-Ali
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
CA-MRSA
lcsh:QR1-502
MRSA
medicine.disease_cause
lcsh:Microbiology
Enterotoxins
Leukocidins
Drug Resistance
Multiple
Bacterial
Genotype
Phylogeny
Cross Infection
Molecular Epidemiology
education.field_of_study
Superantigens
General Medicine
Staphylococcal Infections
Anti-Bacterial Agents
Bacterial Typing Techniques
Community-Acquired Infections
Infectious Diseases
Vancomycin
Female
Infection
MLST
medicine.drug
Adult
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical)
HA-MRSA
Bacterial Toxins
030106 microbiology
Population
Saudi Arabia
SCCmec
Exotoxins
Microbial Sensitivity Tests
Biology
Staphylococcal infections
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Bacterial Proteins
medicine
Humans
Penicillin-Binding Proteins
lcsh:RC109-216
education
Alleles
Molecular epidemiology
Research
lcsh:RM1-950
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
medicine.disease
Methicillin-resistant Staphylococcus aureus
lcsh:Therapeutics. Pharmacology
Genes
Bacterial
Multilocus sequence typing
Carrier
Multilocus Sequence Typing
Zdroj: Annals of Clinical Microbiology and Antimicrobials
Annals of Clinical Microbiology and Antimicrobials, Vol 17, Iss 1, Pp 1-11 (2018)
ISSN: 1476-0711
Popis: Background Methicillin resistant Staphylococcus aureus (MRSA) constitutes a major global health concern causing hospital and community acquired infections. A wide diversity of MRSA genotypes are circulating in geographically related regions. Therefore understanding the molecular epidemiology of MRSA is fundamental to design control and clearance measures. Methods A total of 106 MRSA isolates from infection (51) and carrier colonization sites (55) are characterized genetically based on SCCmec and MLST genotyping methods in addition to detection of PVL, TSST-1 and enterotoxins. Results Sccmec-IV was the most frequently detected genotype (77.3%) followed by genotype V (13.2%) and III (9.4%). SCCmec-IVa was more prevalent among the carrier group (p value 0.002). CC80 was the most commonly identified clonal complex (CC). CC6 and CC22 were significantly more prevalent among the carrier group (p value 0.02 and 0.01, respectively). PVL was highly prevalent among the isolates (58.5%). PVL was detected in 70.6% of isolates from infection sites and 47.3% of isolates from carriers. All strains were sensitive to vancomycin, however, MRSA strains isolated from infection sites had significantly higher MICs compared to strains isolated from carrier colonization sites (p value 0.021). Five new sequence types mainly from the carrier group were identified and described in the study. Conclusions MRSA population is genetically very diverse among carriers and infected individuals. With SCCmec type IV being most prevalent, this suggests a community origin of most MRSA strains. Therefore very well designed surveillance and clearance strategies should be prepared to prevent emergence and control spread of MRSA in the community.
Databáze: OpenAIRE
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