A glycophospholipid anchor is required for Qa-2-mediated T cell activation
| Autor: | Peter Robinson, Elizabeth M. Simpson, Margaret Millrain, Andrew L. Mellor, Jane Antoniou |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
DNA Replication
Genetically modified mouse Glycosylphosphatidylinositols T-Lymphocytes T cell Lymphocyte Restriction Mapping Mice Inbred Strains Mice Transgenic Biology Lymphocyte Activation Phosphatidylinositols Antibodies Cell membrane Mice Species Specificity Antigen medicine Animals Cloning Molecular Multidisciplinary Cell growth Histocompatibility Antigens Class I H-2 Antigens T lymphocyte Cell biology medicine.anatomical_structure Genes Immunology Glycolipids Cell activation |
| Zdroj: | Nature. 342:85-87 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/342085a0 |
| Popis: | A number of lymphocyte surface proteins are anchored in the cell membrane by glycophosphatidyl inositol (known as GPI) linkages instead of hydrophobic protein domains. Treatment of mouse T lymphocytes with antibodies specific for two such proteins, Thy-1 and Ly-6, are known to induce proliferation. We have found that antibodies specific for Qa-2, a GPI-anchored class I histocompatibility antigen, can also activate mouse T cells. To determine whether the GPI-anchor is important for this pathway of cell activation, we produced transgenic mice expressing either normal GPI-anchored Qa-2, or Qa-2 molecules with a membrane-spanning protein domain derived from H-2. Our studies show that only lymphocytes from transgenic mice carrying GPI-anchored forms of Qa-2 can be activated in vitro by Qa-2-specific antibodies. We also show that transgenic mouse T cells expressing a GPI-anchored form of H-2Db can be activated by anti-H-2Db antibodies. These results strongly indicate that the GPI-anchor is critical for this pathway of T cell activation. |
| Databáze: | OpenAIRE |
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