Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

Autor: Laura M. Lechuga, Juan José González-López, Jesús Maldonado, Ana Belén González-Guerrero, Adrián Fernández-Gavela
Přispěvatelé: Generalitat de Catalunya, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III, Consejo Nacional de Ciencia y Tecnología (México), European Commission, Lechuga, Laura M., Lechuga, Laura M. [0000-0001-5187-5358]
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Diagnostics
Volume 10
Issue 10
Diagnostics, Vol 10, Iss 845, p 845 (2020)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 2075-4418
DOI: 10.3390/diagnostics10100845
Popis: This article belongs to the Collection Diagnostic Sensors.
Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene blaCTX-M-15 and the carbapenemase-encoding gene blaNDM-5 We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria.
This research was supported by CERCA program/Generalitat de Catalunya. ICN2 is supported by the Severo Ochoa Program from Spanish MINECO (Grant No. SEV-2017-0706). The VHIR-HUVH is supported by Plan Nacional de I+D+i 2013-2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/0003), co-financed by the European Development Regional Fund “A way to achieve Europe”. Authors acknowledge the support of project ASPIRE (funded by CIBER-BBN). J. Maldonado acknowledges the Mexican National Council for Science and Technology (CONACYT). This work has made use of the Biodeposition and Biodetection Unit from ICTS NANBIOSIS partially supported by MICINN/FEDER (FICTS- 1420- 27).
Databáze: OpenAIRE
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