A novel extraction method for peanut allergenic proteins in chocolate and their detection by a liposome-based lateral flow assay
| Autor: | Thomas R. DeCory, Wlodzimierz Borejsza-Wysocki, Antje J. Baeumner, Richard A. Durst, Hsiao-Wei Wen |
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| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Liposome Chromatography Extraction (chemistry) food and beverages General Chemistry medicine.disease_cause Biochemistry Industrial and Manufacturing Engineering Hexane chemistry.chemical_compound Allergen chemistry Ara h1 medicine Sample preparation Centrifugation Food Science Biotechnology |
| Zdroj: | European Food Research and Technology. 221:564-569 |
| ISSN: | 1438-2385 1438-2377 |
| DOI: | 10.1007/s00217-005-1202-8 |
| Popis: | In this study, conditions for extracting the major peanut allergen (Ara h1) from chocolate were optimized, and the extracted samples were analyzed by a lateral flow assay (LFA) using liposomal nanovesicles. The optimal conditions using peanut-spiked chocolate were found to be extraction with a mixture of phosphate buffered saline and hexane for 30 min at 35 °C. After centrifugation, the buffer portion was treated with insoluble poly(vinylpolypyrrolidone) to remove phenolic compounds, and then analyzed by the LFA. The entire analysis, including sample preparation and LFA, could be easily completed within 2 h, and the detection limit was 158 μg of peanuts/g of chocolate. |
| Databáze: | OpenAIRE |
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