Validation of a photostability indicating method for quantification of furanocoumarins from Brosimum gaudichaudii soft extract
| Autor: | Mariana Cristina de Morais, Osvaldo de Freitas, Leonardo Luiz Borges, Edemilson Cardoso da Conceição, Paulo Henrique Gomes de Almeida, Nayara Luiza Oliveira Ferreira, Rejanne Lima Arruda |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Best fitting
Mamica-de-cadela Vitiligo lcsh:RS1-441 01 natural sciences Bergapten Moraceae lcsh:Pharmacy and materia medica 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine General Pharmacology Toxicology and Pharmaceutics Soft extract Photodegradation Psoralen HPLC-PDA method Chromatography biology 010401 analytical chemistry Biological potential biology.organism_classification Brosimum 0104 chemical sciences chemistry PRODUTOS NATURAIS |
| Zdroj: | Revista Brasileira de Farmacognosia v.28 n.1 2018 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA Revista Brasileira de Farmacognosia, Volume: 28, Issue: 1, Pages: 118-123, Published: JAN 2018 Revista Brasileira de Farmacognosia, Vol 28, Iss 1, Pp 118-123 (2018) Revista Brasileira de Farmacognosia, Vol 28, Iss 1, Pp 118-123 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | A validation study of a reverse-phase high-performance liquid chromatographic assay for the quantification of two furanocoumarins (psoralen and bergapten) in soft extract obtained from Brosimum gaudichaudii Trécul, Moraceae, roots was conducted. The developed method was sensitive, rapid, reproducible, easy and precise, and showed linearity (r > 0.99) in the range of 10–64 μg/ml for psoralen, and 9–56 μg/ml for bergapten. It also showed a good efficiency for the photodegradation analysis of psoralen and bergapten in the soft extract. The photostability results showed that the Higuchi model presented the best fitting to the obtained data. Both chemical markers showed stability over 2.6 days, suggesting potential applications of the extract in obtaining intermediate products from this plant material. Furanocoumarins take around 30 min to be activated by UV light, reaching the maximum biological potential. Thus, the results obtained to the Higuchi model, corresponding to 2.6 days of stability, shows feasibility with future applications of these chemical markers. Keywords: HPLC-PDA method, Psoralen, Bergapten, Vitiligo, Mamica-de-cadela, Moraceae |
| Databáze: | OpenAIRE |
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