Suppression of in vitro lymphocyte stimulation in mice bearing primary moloney sarcoma virus-induced tumors
| Autor: | H. Kirchner, M. Glaser, David H. Lavrin, R.B. Herberman |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Lipopolysaccharides
Male Moloney Sarcoma Virus Lipopolysaccharide viruses Immunology Population Spleen Lymphocyte Activation Virus Mice chemistry.chemical_compound Lectins Concanavalin A Escherichia coli medicine Animals Neoplasm Lymphocytes education Cells Cultured Immunosuppression Therapy education.field_of_study biology Pokeweed mitogen Polysaccharides Bacterial medicine.disease Virology Molecular biology Mice Inbred C57BL medicine.anatomical_structure chemistry biology.protein Sarcoma Experimental Mitogens Moloney murine leukemia virus Methylcholanthrene |
| Zdroj: | Cellular Immunology. 13:32-40 |
| ISSN: | 0008-8749 |
| Popis: | A marked depression of phytohemagglutinin (PHA) reactivity was observed in spleen cell cultures of C57B1/6N mice bearing primary Moloney sarcoma virus (MSV)-induced tumors. This defect was most pronounced 14 days after virus inoculation (MSV 14) and was reversed after regression of the tumor. Spleen cells from mice with primary methylcholanthrene-induced sarcomas were similarly deficient while no such effect was observed during the first weeks after inoculation of Moloney leukemia virus. The responses of MSV 14 spleen cells to Concanavalin A (Con A) were as consistently depressed as those to PHA, but reactivity to bacterial lipopolysaccharide was affected to a lesser degree. Stimulation by pokeweed mitogen (PWM) was not significantly lower in tumor-bearing mice than in control animals. Passage of MSV 14 spleen cells over rayon adherence columns which removed about 75% of the initial cell population led to an almost complete restoration of their PHA and Con A responses on a per cell basis. This may indicate that within MSV 14 spleens, T lymphocytes reactive to PHA and Con A are diluted out by a majority of unreactive cells. However, the possibility also exists that column passage removes a suppressor cell that actively inhibits these responses. |
| Databáze: | OpenAIRE |
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