The differential gene expression profiles of proximal and distal muscle groups are altered in pre-pathological dysferlin-deficient mice
| Autor: | Reginald E. Bittner, Steven H. Laval, Volker Straub, Harald Höger, Kate Bushby, Faye Haldane, Louise V.B. Anderson, Maria Wiedner, LM Cree, Herbert A. Reitsamer, Felicitas Oberndorfer, Ilka Wappler, Heiko Peters, Matthew Pocock, Maja von der Hagen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Dysferlinopathy
Pathology medicine.medical_specialty Time Factors Microarray Blotting Western Gene Expression Muscle Proteins Distal Muscle Muscular Dystrophies Dysferlin Mice Gene expression medicine Animals Calgranulin B RNA Messenger Muscular dystrophy Muscle Skeletal Genetics (clinical) Mice Knockout biology Microarray analysis techniques Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Myocardium Membrane Proteins Reproducibility of Results medicine.disease Microarray Analysis Molecular biology Immunohistochemistry Gene expression profiling Mice Inbred C57BL Disease Models Animal Neurology Pediatrics Perinatology and Child Health biology.protein Mice Inbred mdx Neurology (clinical) |
| Zdroj: | Neuromuscular disorders : NMD. 15(12) |
| ISSN: | 0960-8966 |
| Popis: | The selective pattern of muscle involvement is a key feature of muscular dystrophies. Dysferlinopathy is a good model for studying this process since it shows variable muscle involvement that can be highly selective even in individual patients. The transcriptomes of proximal and distal muscles from wildtype C57BL/10 and dysferlin deficient C57BL/10.SJL-Dysf mice at a prepathological stage were assessed using the Affymetrix oligonucleotide-microarray system. We detected significant variation in gene expression between proximal and distal muscle in wildtype mice. Dysferlin defiency, even in the absence of pathological changes, altered this proximal distal difference but with little specific overlap with previous microarray analyses of dysferlinopathy. In conclusion, proximal and distal muscle groups show distinct patterns of gene expression and respond differently to dysferlin deficiency. This has implications for the selection of muscles for future microarray analyses, and also offers new routes for investigating the selectivity of muscle involvement in muscular dystrophies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect