Cellular uptake of lipoproteins and persistent organic compounds—An update and new data
| Autor: | Philip S. Hjelmborg, Thomas K. Andreassen, Eva Cecilie Bonefeld-Jørgensen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipoproteins
Metabolite Cell Cell Culture Techniques Biochemistry Cell Line Mice chemistry.chemical_compound parasitic diseases medicine Animals Organic Chemicals Receptor General Environmental Science Mice Knockout LDL POPs MEF cells receptor mediated uptake ultracentrifugation Biological Transport Fibroblasts In vitro medicine.anatomical_structure Receptors LDL Mechanism of action chemistry Nuclear receptor LDL receptor Environmental Pollutants lipids (amino acids peptides and proteins) medicine.symptom Ultracentrifugation Lipoprotein |
| Zdroj: | Hjelmborg, P S, Andreassen, T K & Bonefeld-Jørgensen, E C 2008, ' Cellular uptake of lipoproteins and Persistent Organic Compounds-An update and new data ', Environmental Research, vol. 108, no. 2, pp. 192-198 . https://doi.org/10.1016/j.envres.2008.07.019 |
| ISSN: | 0013-9351 |
| DOI: | 10.1016/j.envres.2008.07.019 |
| Popis: | Udgivelsesdato: October 2008 There are a number of interactions related to transport of lipophilic xenobiotic compounds in the blood stream of mammals. This paper will focus on the interactions between lipoproteins and persistent organic pollutants (POPs) and how these particles are taken up by cells. A number of POPs including the pesticide DDT (p,p'-dichlorodiphenyltrichloroethane), and especially its metabolite DDE (p,p'-dichlorodiphenyldichloroethene), interacts with nuclear hormone receptors causing these to malfunction, which in turn results in a range of deleterious health effects in humans.The aim of the present study was to determine the role of lipoprotein receptors in mouse embryonic fibroblast (MEF) cells in conjunction with uptake of DDT-lipoprotein complexes from supplemented media in vitro.Uptake of DDT by MEF cells was investigated using MEF1 cells carrying the receptors LRP (low-density lipoprotein receptor-related protein) and LDLR (low density lipoprotein receptor) present and MEF4 cells with no LRP and LDLR expression. Cells were incubated together with the complex of LDL and [14C]DDT. The receptor function was further evaluated by adding the 40 kDa receptor-associated protein (RAP) which blocks receptor activity. The results showed that [14C]DDT uptake was inversely dependent on the LDL concentration. There was no strong evidence for a receptor mediated uptake of the [14C]DDT-lipoprotein complex. To conclude, DDT travels in the blood stream and can cross cell membranes while being transported as a DDT-lipoprotein complex. The lipoproteins do not need receptors to cross cell membranes since passive diffusion constitutes a major passageway. |
| Databáze: | OpenAIRE |
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