Different Reactive Oxygen Species Lead to Distinct Changes of Cellular Metal Ions in the Eukaryotic Model Organism Saccharomyces cerevisiae
| Autor: | Patricia A Murphy, Melinda Christophersen, Peter J. Rogers, Patrick J. O'Doherty, Ming J. Wu, Trevor D. Bailey, Vincent J. Higgins, Victoria Lyons |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Potassium yeast medicine.disease_cause lcsh:Chemistry chemistry.chemical_compound Menadione Benzene Derivatives Magnesium Hydrogen peroxide lcsh:QH301-705.5 Spectroscopy Diamide chemistry.chemical_classification reactive oxygen species biology Vitamin K 3 General Medicine Oxidants Computer Science Applications Linoleic Acids Biochemistry Saccharomyces cerevisiae Lipid Peroxides chemistry.chemical_element Oxidative phosphorylation Article Catalysis Inorganic Chemistry Peroxynitrous Acid medicine Physical and Theoretical Chemistry Molecular Biology Ions Manganese Reactive oxygen species Cyclohexanones Organic Chemistry metal ions Hydrogen Peroxide biology.organism_classification Yeast Oxidative Stress chemistry lcsh:Biology (General) lcsh:QD1-999 ionomic profiling Copper Oxidative stress Aluminum |
| Zdroj: | International Journal of Molecular Sciences, Vol 12, Iss 11, Pp 8119-8132 (2011) International Journal of Molecular Sciences Volume 12 Issue 11 Pages 8119-8132 |
| ISSN: | 1422-0067 |
| Popis: | Elemental uptake and export of the cell are tightly regulated thereby maintaining the ionomic homeostasis. This equilibrium can be disrupted upon exposure to exogenous reactive oxygen species (ROS), leading to reduction or elevation of the intracellular metal ions. In this study, the ionomic composition in the eukaryotic model organism Saccharomyces cerevisiae was profiled using the inductively-coupled plasma optical emission spectrometer (ICP-OES) following the treatment with individual ROS, including hydrogen peroxide, cumen hydroperoxide, linoleic acid hydroperoxide (LAH), the superoxide-generating agent menadione, the thiol-oxidising agent diamide [diazine-dicarboxylic acid-bis(dimethylamide)], dimedone and peroxynitrite. The findings demonstrated that different ROS resulted in distinct changes in cellular metal ions. Aluminium (Al(3+)) level rose up to 50-fold after the diamide treatment. Cellular potassium (K(+)) in LAH-treated cells was 26-fold less compared to the non-treated controls. The diamide-induced Al(3+) accumulation was further validated by the enhanced Al(3+) uptake along the time course and diamide doses. Pre-incubation of yeast with individual elements including iron, copper, manganese and magnesium failed to block diamide-induced Al(3+) uptake, suggesting Al(3+)-specific transporters could be involved in Al(3+) uptake. Furthermore, LAH-induced potassium depletion was validated by a rescue experiment in which addition of potassium increased yeast growth in LAH-containing media by 26% compared to LAH alone. Taken together, the data, for the first time, demonstrated the linkage between ionomic profiles and individual oxidative conditions. |
| Databáze: | OpenAIRE |
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