Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives
| Autor: | Irena Novaković, Dušan Sladić, Miroslav J. Gašić, Mario Zlatović, Uroš Anđelković |
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| Rok vydání: | 2012 |
| Předmět: |
Gram-negative bacteria
Stereochemistry Biomedical Engineering Pharmaceutical Science Bioengineering Microbial Sensitivity Tests Molecular Dynamics Simulation Sesquiterpene 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Residue (chemistry) Cyclohexenes Organic chemistry 030304 developmental biology Pharmacology chemistry.chemical_classification 0303 health sciences biology Bacteria 010405 organic chemistry Chemistry Organic Chemistry Temperature biology.organism_classification 0104 chemical sciences Quinone Anti-Bacterial Agents Enzyme Activation Enzyme Muramidase Lysozyme Antibacterial activity Sesquiterpenes Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry |
| Popis: | A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates. This is the peer-reviewed version of the article: Novaković, I., Anđelković, U., Zlatović, M., Gašić, M.J., Sladić, D., 2012. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. Bioconjugate Chem. 23, 57–65. [https://doi.org/10.1021/bc200330m] |
| Databáze: | OpenAIRE |
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