Development and evaluation of a blocking enzyme-linked immunosorbent assay and virus neutralization assay to detect antibodies to viral hemorrhagic septicemia virus
| Autor: | Anna Wilson, Frederick W. Goetz, Lucas M. Hart, Tony L. Goldberg, Wendy J. Olson, Susan V. Marcquenski, Paul K. Hershberger, Kathy Toohey-Kurth |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Veterinary Medicine Virus isolation Clinical Biochemistry Immunology Virus Neutralization Enzyme-Linked Immunosorbent Assay Antibodies Viral Sensitivity and Specificity Virus Serology Novirhabdovirus Fish Diseases Neutralization Tests Diagnostic Laboratory Immunology Hemorrhagic Septicemia Viral Immunology and Allergy Animals Serologic Tests chemistry.chemical_classification biology biology.organism_classification Virology United States Enzyme chemistry biology.protein Viral hemorrhagic septicemia Antibody |
| Zdroj: | Clinical and vaccine immunology : CVI. 21(3) |
| ISSN: | 1556-679X |
| Popis: | Viral hemorrhagic septicemia virus (VHSV) is a target of surveillance by many state and federal agencies in the United States. Currently, the detection of VHSV relies on virus isolation, which is lethal to fish and indicates only the current infection status. A serological method is required to ascertain prior exposure. Here, we report two serologic tests for VHSV that are nonlethal, rapid, and species independent, a virus neutralization (VN) assay and a blocking enzyme-linked immunosorbent assay (ELISA). The results show that the VN assay had a specificity of 100% and sensitivity of 42.9%; the anti-nucleocapsid-blocking ELISA detected nonneutralizing VHSV antibodies at a specificity of 88.2% and a sensitivity of 96.4%. The VN assay and ELISA are valuable tools for assessing exposure to VHSV. |
| Databáze: | OpenAIRE |
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