Inhibition of pancreatic β-cell glucokinase by antisense RNA expression in transgenic mice: mouse strain-dependent alteration of glucose tolerance
Autor: | Hideki Katagiri, Junichi Miyazaki, Tomoichiro Asano, Koichi Ikuta, Fumi Tashiro, Yoshio Yazaki, Yoshitomo Oka, Hisamitsu Ishihara, Koichi Inukai, Masatoshi Kikuchi |
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Rok vydání: | 1995 |
Předmět: |
Blood Glucose
Genetically modified mouse medicine.medical_specialty Antisense mRNA Monosaccharide Transport Proteins Transgene medicine.medical_treatment Cell Biophysics Muscle Proteins Mice Transgenic Biology Glucose homeostasis Biochemistry Islets of Langerhans Mice Transgenic mouse Structural Biology Internal medicine Glucokinase Insulin Secretion Genetics medicine Animals Insulin RNA Antisense RNA Messenger Phosphorylation Muscle Skeletal Molecular Biology Mice Inbred C3H Messenger RNA Glucose Transporter Type 4 Cell Biology Glucose Tolerance Test Antisense RNA Mice Inbred C57BL Glucose medicine.anatomical_structure Endocrinology Gene Expression Regulation Mice Inbred DBA |
Zdroj: | FEBS Letters. 371:329-332 |
ISSN: | 0014-5793 |
Popis: | We have generated transgenic mice, in either C57BL/6 or C3H background, expressing antisense glucokinase mRNA in β-cells. The glucose phosphorylating activity at 60 mM glucose in transgenic islets was significantly lower than that in controls, and the insulin secretory response to glucose was lower in transgenic islets than in those of controls in both strains. Following i.p. glucose challenge, higher blood glucose levels were observed in transgenic mice than in controls in the C57BL/6 nut not the C3H background. These data suggest that a β-cell secretory defect, in combination with other undefined genetic factors, causes impaired glucose homeostasis in mice. |
Databáze: | OpenAIRE |
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