PCBP2 promotes the development of glioma by regulating FHL3/TGF‐β/Smad signaling pathway
| Autor: | Jianhui Wei, Yongjian Cui, Lei Zheng, Jianhui Mao, Hong Guo, Naiyi Du, Zhaosheng Sun, Zhenmin Hao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
FHL3 Male Physiology Clinical Biochemistry Apoptosis SMAD Smad7 Protein Transforming Growth Factor beta1 03 medical and health sciences Mice 0302 clinical medicine TGF‐β/Smad pathway Glioma Original Research Articles Cell Line Tumor medicine Gene silencing Animals Humans Original Research Article Gene Silencing PCBP2 Cell Proliferation Gene knockdown Chemistry Intracellular Signaling Peptides and Proteins RNA-Binding Proteins Cell Biology Transfection LIM Domain Proteins Middle Aged medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology Lipofectamine 030220 oncology & carcinogenesis Cancer research Heterografts Female Signal transduction Transforming growth factor Signal Transduction |
| Zdroj: | Journal of Cellular Physiology |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | The purpose of this study was to investigate the role of Poly (C)‐binding protein 2 (PCBP2) and the related signaling pathway in glioma progression. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and immunohistochemistry (IHC) were performed to measure PCBP2 messenger RNA and protein expression in glioma tissues or cells. Cell transfection was completed using Lipofectamine 2000. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, Transwell assay and flow cytometry assay were used to explore the effects of PCBP2 expression on biological behaviors of glioma cells. Western blot assay was used for the detection of pathway related proteins. Expression of PCBP2 in glioma tissues and cells were higher than that in paracancerous tissues and normal cells (both p Poly (C)‐binding protein 2 (PCBP2) activates TGF‐β/Smad signaling pathway by inhibiting FHL3 expression, thus promoting the development and progression of glioma. |
| Databáze: | OpenAIRE |
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