Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males
| Autor: | Kevin D. Cashman, Fiona Moloney, Anne Mullen, Lorna Doyle, Helen M. Roche, Anne P. Nugent |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Interleukin 2
Adult Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Linoleic acid Conjugated linoleic acid medicine.medical_treatment Clinical Biochemistry Biology Fibrinogen Biochemistry Peripheral blood mononuclear cell chemistry.chemical_compound Immune system Double-Blind Method Internal medicine medicine Concanavalin A Humans Linoleic Acids Conjugated Molecular Biology Nutrition and Dietetics integumentary system Tumor Necrosis Factor-alpha food and beverages Interleukin Middle Aged Interleukin-10 Endocrinology Cytokine chemistry Immunology Leukocytes Mononuclear Interleukin-2 lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | The Journal of nutritional biochemistry. 18(10) |
| ISSN: | 0955-2863 |
| Popis: | Conjugated linoleic acid (CLA) refers to geometric and positional isomers of linoleic acid. Animal studies have shown that CLA modulates the immune system and suggest that it may have a therapeutic role in inflammatory disorders. This double-blind placebo-controlled intervention trial investigated the effects of CLA supplementation on indices of immunity relating to cardiovascular disease (CVD) in a cohort of healthy middle-aged male volunteers. Subjects were randomly assigned to supplement their diet with 2.2 g 50:50 isomeric blend of cis 9, trans 11 (c9, t11)-CLA and trans 10, cis 12 (t10, c12)-CLA or placebo daily for 8 weeks. Interleukin (IL) 2, IL-10 and tumour necrosis factor (TNF) alpha were measured in the supernatant of cultured unstimulated and concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC) by ELISA. Serum IL-6 and plasma CRP were measured by ELISA and plasma fibrinogen by automated clotting assay. Gene expression was investigated by real-time RT-PCR. CLA supplementation significantly reduced Con A-stimulated PBMC IL-2 secretion (37.1%; P=.02). CLA supplementation had no significant effect on transcription of IL-2. CLA supplementation had no direct significant effects on PBMC TNFalpha or IL-10 secretion. Other inflammatory markers associated with CVD, including IL-6, CRP and fibrinogen, were not affected by CLA supplementation. This study showed that CLA supplementation reduced PBMC IL-2 secretion from Con A-stimulated PBMC but lacked effect on other markers of the human inflammatory response. |
| Databáze: | OpenAIRE |
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