The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae)

Autor: Taila Andrade Lemos, Ana Maria Lima de Azeredo-Espin, F. R. da Silva, André Luiz Vettore, Paulo Arruda, Edson L. Kemper, A. C. Martins Junqueira, Ana Cláudia Lessinger
Rok vydání: 2000
Předmět:
Zdroj: Insect Molecular Biology. 9:521-529
ISSN: 1365-2583
0962-1075
Popis: The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16,022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.
Databáze: OpenAIRE