Plant stress granules and mRNA processing bodies are distinct from heat stress granules

3' mRNA degradation pathway, and eIF4E, as well as the RNA binding proteins RBP47 and UBP1, as marker proteins for stored mRNPs in SGs. Cycloheximide-inhibited translation, stress treatments and mutants defective in mRNP homeostasis were used to study the dynamic transitions of mRNPs between SGs and PBs. SGs and PBs can be clearly discriminated from the previously described heat stress granules (HSGs), which evidently do not contain mRNPs. Thus, the role of HSGs as putative mRNP storage sites must be revised. -->

ISSN:	1365-313X 0960-7412
DOI:	10.1111/j.1365-313x.2008.03623.x
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee42e2cabe4d6873e8c07507b6dd8078 https://doi.org/10.1111/j.1365-313x.2008.03623.x
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....ee42e2cabe4d6873e8c07507b6dd8078
Autor:	Markus Fauth, Lutz Nover, Christian Weber
Rok vydání:	2008
Předmět:	RNA Stability Arabidopsis RNA-binding protein Plant Science Biology Cytoplasmic Granules Stress granule stomatognathic system Stress Physiological Polysome Endoribonucleases P-bodies Genetics Cycloheximide Cells Cultured Plant Proteins Ribonucleoprotein Messenger RNA Arabidopsis Proteins Protoplasts fungi RNA-Binding Proteins Translation (biology) Cell Biology Cell biology Eukaryotic Initiation Factor-4E Ribonucleoproteins Cytoplasm Polyribosomes Protein Biosynthesis Exoribonucleases
Zdroj:	The Plant Journal. 56:517-530
ISSN:	1365-313X 0960-7412
DOI:	10.1111/j.1365-313x.2008.03623.x
Popis:	Similar to the situation in mammalian cells and yeast, messenger ribonucleo protein (mRNP) homeostasis in plant cells depends on rapid transitions between three functional states, i.e. translated mRNPs in polysomes, stored mRNPs and mRNPs under degradation. Studies in mammalian cells showed that whenever the dynamic exchange of the components between these states is disrupted, stalled mRNPs accumulate in cytoplasmic aggregates, such as stress granules (SGs) or processing bodies (PBs). We identified PBs and SGs in plant cells by detection of DCP1, DCP2 and XRN4, as marker proteins for the 5'-->3' mRNA degradation pathway, and eIF4E, as well as the RNA binding proteins RBP47 and UBP1, as marker proteins for stored mRNPs in SGs. Cycloheximide-inhibited translation, stress treatments and mutants defective in mRNP homeostasis were used to study the dynamic transitions of mRNPs between SGs and PBs. SGs and PBs can be clearly discriminated from the previously described heat stress granules (HSGs), which evidently do not contain mRNPs. Thus, the role of HSGs as putative mRNP storage sites must be revised.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee42e2cabe4d6873e8c07507b6dd8078 https://doi.org/10.1111/j.1365-313x.2008.03623.x Zobrazit plný text záznamu Plný text