Endothelial von Willebrand factor recruits platelets to atherosclerosis-prone sites in response to hypercholesterolemia
| Autor: | Desire Collen, Ingrid Vreys, Gregor Theilmeier, Carine Michiels, Erik Spaepen, Jos Vermylen, Marc Hoylaerts |
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| Rok vydání: | 2002 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Endothelium P-selectin Arteriosclerosis Immunology Hypercholesterolemia Cell Communication Platelet Membrane Glycoproteins Platelet membrane glycoprotein Biochemistry Platelet Adhesiveness Von Willebrand factor Cell Movement Platelet adhesiveness Internal medicine von Willebrand Factor medicine Animals Platelet Aorta biology Platelet Glycoprotein GPIb-IX Complex Cell Biology Hematology Disease Models Animal P-Selectin medicine.anatomical_structure Endocrinology biology.protein Endothelium Vascular Rabbits Stress Mechanical Ex vivo |
| Zdroj: | Blood. 99(12) |
| ISSN: | 0006-4971 |
| Popis: | Platelets are thought to play a causal role during atherogenesis. Platelet-endothelial interactions in vivo and their molecular mechanisms under shear are, however, incompletely characterized. Here, an in vivo platelet homing assay was used in hypercholesterolemic rabbits to track platelet adhesion to plaque predilection sites. The role of platelet versus aortic endothelial cell (EC) activation was studied in an ex vivo flow chamber. Pathways of human platelet immobilization were detailed during in vitro perfusion studies. In rabbits, a 0.125% cholesterol diet induced no lesions within 3 months, but fatty streaks were found after 12 months. ECs at segmental arteries of 3- month rabbits expressed more von Willebrand factor (VWF) and recruited 5-fold more platelets than controls (P |
| Databáze: | OpenAIRE |
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