Anaplasma marginale major surface protein 3 is encoded by a polymorphic, multigene family
| Autor: | L E Perryman, A. R. Alleman, Anthony F. Barbet, Travis C. McGuire, Guy H. Palmer, Terry F. McElwain |
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| Rok vydání: | 1997 |
| Předmět: |
Anaplasma
Sequence analysis Molecular Sequence Data Restriction Mapping Immunology Gene Dosage Biology Microbiology Genome Gene dosage Conserved sequence Genetic Heterogeneity Bacterial Proteins parasitic diseases Antigenic variation Amino Acid Sequence Gene Peptide sequence Conserved Sequence Genetics Antigens Bacterial Polymorphism Genetic Base Sequence Nucleic acid sequence Chromosomes Bacterial Recombinant Proteins Infectious Diseases Genes Bacterial Multigene Family Parasitology Research Article |
| Zdroj: | Infection and Immunity. 65:156-163 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.65.1.156-163.1997 |
| Popis: | The immunodominant surface protein, MSP3, is structurally and antigenically polymorphic among strains of Anaplasma marginale. In this study we show that a polymorphic multigene family is at least partially responsible for the variation seen in MSP3. The A. marginale msp3 gene msp3-12 was cloned and expressed in Escherichia coli. With msp3-12 as a probe, multiple, partially homologous gene copies were identified in the genomes of three A. marginale strains. These copies were widely distributed throughout the chromosome. Sequence analysis of three unique msp3 genes, msp3-12, msp3-11, and msp3-19, revealed both conserved and variant regions within the open reading frames. Importantly, msp3 contains amino acid blocks related to another polymorphic multigene family product, MSP2. These data, in conjunction with data presented in previous studies, suggest that multigene families are used to vary important antigenic surface proteins of A. marginale. These findings may provide a basis for studying antigenic variation of the organism in persistently infected carrier cattle. |
| Databáze: | OpenAIRE |
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