Changes in tubular basolateral membrane markers after chronic DOCA treatment
| Autor: | A. Doucet, F. Le Bouffant, François Morel, M. Imbert-Teboul, G. El Mernissi, S. Siaume, Annette Hus-Citharel, M. Montegut, D. Chabardès |
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| Rok vydání: | 1983 |
| Předmět: |
Calcitonin
Male medicine.medical_specialty Physiology medicine.medical_treatment Adenylate kinase Nephron Cyclase Steroid Internal medicine medicine Animals Distal convoluted tubule Desoxycorticosterone Kidney Tubules Distal Epithelial polarity Adenosine Triphosphatases urogenital system Chemistry Cell Membrane Sodium Nephrons Kidney Tubules medicine.anatomical_structure Tubule Endocrinology Potassium Ca(2+) Mg(2+)-ATPase Rabbits Sodium-Potassium-Exchanging ATPase Adenylyl Cyclases Hormone |
| Zdroj: | American Journal of Physiology-Renal Physiology. 245:F100-F109 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.1983.245.1.f100 |
| Popis: | Chronic administration of DOCA to rabbits is known to increase the surface area of the basolateral membrane and the Na-K-ATPase activity of the cortical collecting tubule (CCT). We attempted to ascertain 1) whether Na-K-ATPase is the only basolateral membrane marker induced by DOCA, and 2) whether CCT is the only nephron segment affected by this steroid. We measured the activity of Na-K-ATPase and adenylate cyclase (AC) and the protein content of nephron segments microdissected from control and DOCA-treated rabbits. Morphogenic effects of DOCA, assessed by 30-60% increases in protein content, were specifically observed in the distal convoluted tubule, CCT, and medullary collecting tubule. When expressed as a function of tubular length, Na-K-ATPase activity rose from 80 to 200% in all these segments, whereas the increments in AC of 40-70%, observed in response to four different hormones, occurred only in some of them. When expressed as a function of protein content, Na-K-ATPase activity increased but AC activity remained unchanged. This study indicates that the morphogenic action resulting from chronic DOCA administration affects the entire rabbit distal nephron. During this action Na-K-ATPase is the preferentially induced enzyme. |
| Databáze: | OpenAIRE |
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