Growth suppression of MCF-7 cancer cell-derived xenografts in nude mice by caveolin-1
| Autor: | Baoju Qi, Ping Wu, Xiao-Hui Wang, Yeqing Cui, Hua Zhu, Fei Li, Jianwen Chen, Shuang Liu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Caveolin 1 Biophysics Mice Nude Apoptosis Breast Neoplasms Adenocarcinoma Biology Transfection medicine.disease_cause Biochemistry Mice Cell Movement Cell Line Tumor Internal medicine medicine Animals Humans Genes Tumor Suppressor Neoplasm Invasiveness skin and connective tissue diseases Molecular Biology Cell Proliferation Cell growth Cell Biology medicine.disease Xenograft Model Antitumor Assays Endocrinology Tumor progression Cell culture Cancer cell Cancer research Carcinogenesis |
| Zdroj: | Biochemical and Biophysical Research Communications. 376:215-220 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.08.146 |
| Popis: | Caveolin-1 is an essential structural constituent of caveolae membrane domains that has been implicated in mitogenic signaling and oncogenesis. However, the exact functional role of caveolin-1 still remains controversial. In this report, utilizing MCF-7 human breast adenocarcinoma cells stably transfected with caveolin-1 (MCF-7/cav-1 cells), we demonstrate that caveolin-1 expression dramatically inhibits invasion and migration of these cells. Importantly, in vivo experiments employing xenograft tumor models demonstrated that expression of caveolin-1 results in significant growth inhibition of breast tumors. Moreover, a dramatic delay in tumor progression was observed in MCF-7/cav-1 cells as compared with MCF-7 cells. Histological analysis of tumor sections demonstrated a marked decrease in the percentage of proliferating tumor cells (Ki-67 assay) along with an increase in apoptotic tumor cells (TUNEL assay) in MCF-7/cav-1-treated animals. Our current findings provide for the first time in vivo evidence that caveolin-1 can indeed function as a tumor suppressor in human breast adenocarcinoma derived from MCF-7 cells rather than as a tumor promoter. |
| Databáze: | OpenAIRE |
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