Clinical manifestations of dengue in relation to dengue serotype and genotype in Malaysia: A retrospective observational study

Autor: Ravindran Thayan, Syafinaz Amin-Nordin, Naematul Ain Ahmad-Najimudin, Manisya Zauri Abdul-Wahid, Gary Kim Kuan Low, Lailatul Akmar Mat-Nor, Hui Yee Chee, Siew Mooi Ching, Jeyanthi Suppiah
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Serotype
myalgia
Male
RNA viruses
Viral Diseases
Genotyping Techniques
Artificial Gene Amplification and Extension
Dengue virus
medicine.disease_cause
Pathology and Laboratory Medicine
Polymerase Chain Reaction
Dengue fever
Dengue Fever
Dengue
Tertiary Care Centers
Geographical Locations
0302 clinical medicine
Genotype
Medicine and Health Sciences
030212 general & internal medicine
Young adult
Child
Phylogeny
Data Management
Geography
Reverse Transcriptase Polymerase Chain Reaction
lcsh:Public aspects of medicine
virus diseases
Arthralgia
Phylogenetics
Phylogeography
Infectious Diseases
Biogeography
Medical Microbiology
Viral Pathogens
Viruses
Female
medicine.symptom
Pathogens
Research Article
Neglected Tropical Diseases
Computer and Information Sciences
Asia
lcsh:Arctic medicine. Tropical medicine
Adolescent
lcsh:RC955-962
030231 tropical medicine
Pain
Real-Time Polymerase Chain Reaction
Serogroup
Research and Analysis Methods
Microbiology
03 medical and health sciences
Young Adult
Signs and Symptoms
Diagnostic Medicine
medicine
Genetics
Humans
Pain Management
Evolutionary Systematics
Serotyping
Molecular Biology Techniques
Genotyping
Microbial Pathogens
Molecular Biology
Retrospective Studies
Taxonomy
Evolutionary Biology
Biology and life sciences
Flaviviruses
Population Biology
business.industry
Ecology and Environmental Sciences
Public Health
Environmental and Occupational Health
Malaysia
Organisms
Retrospective cohort study
lcsh:RA1-1270
Sequence Analysis
DNA
Myalgia
Dengue Virus
medicine.disease
Tropical Diseases
Virology
People and Places
Earth Sciences
business
Population Genetics
Zdroj: PLoS Neglected Tropical Diseases, Vol 12, Iss 9, p e0006817 (2018)
PLoS Neglected Tropical Diseases
ISSN: 1935-2735
1935-2727
Popis: Background Malaysia experienced an unprecedented dengue outbreak from the year 2014 to 2016 that resulted in an enormous increase in the number of cases and mortality as compared to previous years. The causes that attribute to a dengue outbreak can be multifactorial. Viral factors, such as dengue serotype and genotype, are the components of interest in this study. Although only a small number of studies investigated the association between the serotype of dengue virus and clinical manifestations, none of these studies included analyses on dengue genotypes. The present study aims to investigate dengue serotype and genotype-specific clinical characteristics among dengue fever and severe dengue cases from two Malaysian tertiary hospitals between 2014 and mid-2017. Methodology and principal findings A total of 120 retrospective dengue serum specimens were subjected to serotyping and genotyping by Taqman Real-Time RT-PCR, sequencing and phylogenetic analysis. Subsequently, the dengue serotype and genotype data were statistically analyzed for 101 of 120 corresponding patients’ clinical manifestations to generate a descriptive relation between the genetic components and clinical outcomes of dengue infected patients. During the study period, predominant dengue serotype and genotype were found to be DENV 1 genotype I. Additionally, non-severe clinical manifestations were commonly observed in patients infected with DENV 1 and DENV 3. Meanwhile, patients with DENV 2 infection showed significant warning signs and developed severe dengue (p = 0.007). Cases infected with DENV 2 were also commonly presented with persistent vomiting (p = 0.010), epigastric pain (p = 0.018), plasma leakage (p = 0.004) and shock (p = 0.038). Moreover, myalgia and arthralgia were highly prevalent among DENV 3 infection (p = 0.015; p = 0.014). The comparison of genotype-specific clinical manifestations showed that DENV 2 Cosmopolitan was significantly common among severe dengue patients. An association was also found between genotype I of DENV 3 and myalgia. In a similar vein, genotype III of DENV 3 was significantly common among patients with arthralgia. Conclusion The current data contended that different dengue serotype and genotype had caused distinct clinical characteristics in infected patients.
Author summary The study highlights interesting relationship between viral factors and clinical manifestation of dengue disease during an outbreak. The viral factors which include serotype and genotype of dengue virus were studied to discover if the clinical manifestation in patients were serotype and genotype-specific. As most clinical symptoms of severe dengue infection only manifest at a much later stage of dengue infection, therefore, information on serotype or genotype-specific dengue manifestations may serve as early surrogate markers to predict disease progression. We found that specific clinical manifestations were over-represented by a specific DENV serotype and genotype. Severe dengue was significantly present in DENV 2 Cosmopolitan-infected group while non-severe dengue was prominent among DENV 1 genotype I-infected patients. DENV 3-infected patients commonly manifested musculoskeletal symptoms. This study was undertaken between 2014 and mid-2017, which was considered to be crucial, given that Malaysia experienced an unprecedented outbreak of dengue during this period. Consequently, the occurrence of serotype shift and possible re-emergence of DENV 3 genotype I were reported.
Databáze: OpenAIRE
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