Genomic diversity in a population of Spodoptera frugiperda nucleopolyhedrovirus
| Autor: | José María Niz, Víctor Romanowski, Marcelo Facundo Berretta, María Leticia Ferrelli, Matías Luis Pidre, María Laura Fabre, Tomás Masson |
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| Rok vydání: | 2020 |
| Předmět: |
Bioquímica
0301 basic medicine Microbiology (medical) Nonsynonymous substitution 030106 microbiology Population Argentina Genomics Context (language use) Genome Viral Spodoptera Microbiology Genome diversity 03 medical and health sciences baculovirus Molecular evolution evolution genomics Genetics SfMNPV Coding region Animals education Molecular Biology Ciencias Exactas Ecology Evolution Behavior and Systematics education.field_of_study Genetic diversity biology fungi biology.organism_classification Nucleopolyhedroviruses 030104 developmental biology Infectious Diseases Larva |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 90 |
| ISSN: | 1567-7257 |
| Popis: | Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) represents a strong candidate to develop environmental-friendly pesticides against the fall armyworm (Spodoptera frugiperda), a widespread pest that poses a severe threat to different crops around the world. However, little is known regarding the genomic diversity present inside SfMNPV isolates and how it shapes the interactions between virus and host. Here, the genomic diversity present inside an isolate of SfMNPV was explored using high-throughput sequencing for the first time. We identified 704 intrahost single nucleotide variants, from which 184 are nonsynonymous mutations distributed among 82 different coding sequences. We detected several structural variants affecting SfMNPV genome, including two previously reported deletions inside the egt region. A comparative analysis between polymorphisms present in different SfMNPV isolates and our intraisolate diversity data suggests that coding regions with higher genetic diversity are associated with oral infectivity or unknown functions. In this context, through molecular evolution studies we provide evidence of diversifying selection acting on sf29, a putative collagenase which could contribute to the oral infectivity of SfMNPV. Overall, our results contribute to deepen our understanding of the coevolution between SfMNPV and the fall armyworm and will be useful to improve the applicability of this virus as a biological control agent. Instituto de Biotecnologia y Biologia Molecular |
| Databáze: | OpenAIRE |
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