Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal
| Autor: | Paula Bóveda, Antonio López-Sebastián, María Gemma Millán de la Blanca, Berenice Bernal, Eva Martínez-Nevado, Milagros C. Esteso, Juncal García García, Julián Santiago-Moreno, Cristina Castaño, Adolfo Toledano-Díaz, Lucía Martínez-Fresneda |
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| Přispěvatelé: | Fundación Parques Reunidos, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Millán de la Blanca, María Gemma [0000-0002-2810-8510], Martínez-Nevado, Eva [0000-0002-8994-4205], Castaño, Cristina [0000-0003-1134-1436], Bernal, Berenice [0000-0001-9144-1554], Toledano-Díaz, Adolfo [0000-0001-6679-485X], Esteso, Milagros Cristina [0000-0002-8963-5736], Bóveda, Paula [0000-0001-5749-4345], Martínez-Fresneda, Lucía [0000-0002-0967-8593], López-Sebastián, Antonio [0000-0001-8695-7441], Santiago-Moreno, Julián [0000-0001-5551-8120], Millán de la Blanca, María Gemma, Martínez-Nevado, Eva, Castaño, Cristina, Bernal, Berenice, Toledano-Díaz, Adolfo, Esteso, Milagros Cristina, Bóveda, Paula, Martínez-Fresneda, Lucía, López-Sebastián, Antonio, Santiago-Moreno, Julián |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cryoprotectant
Semen cryopreservation Cryopreservation Andrology spermatozoa lcsh:Zoology lcsh:QL1-991 Acrosome freezing/thawing Seminal plasma Sperm motility lcsh:Veterinary medicine General Veterinary Chemistry Morphometry cryoprotectant Spermatozoa Semen cryopreservation Sperm American flamingo seminal plasma Freezing/thawing DNA fragmentation lcsh:SF600-1100 Animal Science and Zoology morphometry |
| Zdroj: | Animals, Vol 11, Iss 203, p 203 (2021) Animals Volume 11 Issue 1 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2076-2615 |
| Popis: | The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen&ndash thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µ m/s vs. 16.64 µ m/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01) but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos seminal plasma removal did not provide a benefit for sperm cryopreservation. |
| Databáze: | OpenAIRE |
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