Investigation of C-reactive protein and AIM2 methylation as a marker for PTSD in Australian Vietnam veterans
| Autor: | Graham Cooksley, John N. Gibson, Rebecca Mellor, Sarah McLeay, Miriam Dwyer, Darrell H. G. Crawford, Ross McD. Young, David Colquhoun, Robyn O'Sullivan, Joanne Voisey, Rachel Thomson, C. R. Strakosch, Madeline Romaniuk, Charles P. Morris, Bruce R. Lawford, Wendy Harvey |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Oncology medicine.medical_specialty Nerve Tissue Proteins Single-nucleotide polymorphism Inflammation Polymorphism Single Nucleotide behavioral disciplines and activities Epigenesis Genetic Vietnam Conflict Proinflammatory cytokine Stress Disorders Post-Traumatic Internal medicine mental disorders Genetics medicine Humans Epigenetics Genetic Association Studies Aged Veterans biology C-reactive protein Australia General Medicine Methylation DNA Methylation Up-Regulation DNA-Binding Proteins C-Reactive Protein Case-Control Studies Cohort DNA methylation biology.protein medicine.symptom |
| Zdroj: | Gene. 803:145898 |
| ISSN: | 0378-1119 |
| Popis: | Chronic inflammation is a key factor in symptomology and comorbidities of post-traumatic stress disorder (PTSD). Levels of a proinflammatory marker, C-reactive protein (CRP) are increased in individuals with PTSD but it is not clear if this is due to trauma exposure or PTSD. Our study aimed to assess the relationship between serum CRP levels, CRP SNPs, methylation, mRNA expression and PTSD in a homogenous trauma exposed Australian Vietnam veteran cohort. We hypothesized that decreased DNA methylation would be associated with increased gene expression and increased peripheral CRP levels in PTSD patients and that this would be independent of trauma. Participants were 299 Vietnam veterans who had all been exposed to trauma and approximately half were diagnosed with PTSD. We observed higher levels of serum CRP in the PTSD group compared to the non-PTSD group but after controlling for BMI and triglycerides the association did not remain significant. No association was found between CRP SNPs and PTSD or CRP levels. Absent in Melanoma 2 (AIM2) which is a mediator of inflammatory response and a determinant of CRP levels was analysed for DNA methylation and mRNA expression. We observed a trend level association between PTSD and AIM2 methylation after controlling for age, smoking, triglycerides, BMI and cell types. There was no significant interaction between PTSD and CRP levels on AIM2 methylation after controlling for covariates. We observed that as AIM2 methylation levels decreased, AIM2 mRNA expression increased. Elevated CRP levels were associated with AIM2 mRNA in the trauma exposed cohort but there was no significant interaction effect with PTSD. Our results could not confirm that CRP is a marker of PTSD independent of trauma in this group of older veterans. CRP may be a broad marker of disease risk, or a marker of PTSD in younger cohorts than those in this study. |
| Databáze: | OpenAIRE |
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