Dual transcript and protein quantification in a massive single cell array
| Autor: | Qiong Pan, Seung-min Park, Luke P. Lee, Parag Mallick, Ivan Dimov, Susie Suh, Anna M. Wu, Keyu Li, Shannon M. Mumenthaler, Hojae Lee, Sang Hun Lee, Jae Young Lee, SoonGweon Hong |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
1.1 Normal biological development and functioning Messenger Cell Quantitative proteomics Biomedical Engineering Bioengineering Biology Biochemistry Article Cell Line Analytical Chemistry 03 medical and health sciences Engineering Underpinning research Cell Line Tumor Gene expression Genetics medicine Humans RNA Messenger Lung Cancer Messenger RNA Tumor Cellular array Lung Cancer Nsclc cell Proteins RNA General Chemistry Cell biology 030104 developmental biology medicine.anatomical_structure Tissue Array Analysis Cell culture Chemical Sciences Generic health relevance Single-Cell Analysis Biotechnology |
| Zdroj: | Lab on a chip, vol 16, iss 19 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | Recently, single-cell molecular analysis has been leveraged to achieve unprecedented levels of biological investigation. However, a lack of simple, high-throughput single-cell methods has hindered in-depth population-wide studies with single-cell resolution. We report a microwell-based cytometric method for simultaneous measurements of gene and protein expression dynamics in thousands of single cells. We quantified the regulatory effects of transcriptional and translational inhibitors on cMET mRNA and cMET protein in cell populations. We studied the dynamic responses of individual cells to drug treatments, by measuring cMET overexpression levels in individual non-small cell lung cancer (NSCLC) cells with induced drug resistance. Across NSCLC cell lines with a given protein expression, distinct patterns of transcript-protein correlation emerged. We believe this platform is applicable for interrogating the dynamics of gene expression, protein expression, and translational kinetics at the single-cell level - a paradigm shift in life science and medicine toward discovering vital cell regulatory mechanisms. |
| Databáze: | OpenAIRE |
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